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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Identification of bovine CpG SNPs as potential targets for epigenetic regulation via DNA methylation

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Autor(es):
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Maldonado, Mariangela B. C. [1] ; de Rezende Neto, Nelson B. [2] ; Nagamatsu, Sheila T. [3, 4] ; Carazzolle, Marcelo F. [4, 5] ; Hoff, Jesse L. [6] ; Whitacre, Lynsey K. [6] ; Schnabel, Robert D. [6, 7] ; Behura, Susanta K. [6] ; McKay, Stephanie D. [8] ; Taylor, Jeremy F. [6] ; Lopes, Flavia L. [1, 9]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Sch Vet Med, Aracatuba, SP - Brazil
[2] ABC Fed Univ, Nat & Human Sci Ctr, Sao Paulo, SP - Brazil
[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Bioethanol Sci & Technol Lab CTBE, Campinas, SP - Brazil
[4] Univ Estadual Campinas, Genom & Express Lab, Campinas, SP - Brazil
[5] Univ Estadual Campinas, Natl Ctr High Performance Comp CENAPAD SP, Campinas, SP - Brazil
[6] Univ Missouri, Div Anim Sci, Columbia, MO - USA
[7] Univ Missouri, Informat Inst, Columbia, MO - USA
[8] Univ Vermont, Dept Anim & Vet Sci, Burlington, VT - USA
[9] Univ Estadual Paulista, UNESP, Sch Vet Med, Rua Clovis Pestana, 793 Jd Dona Amelia, Aracatuba, SP - Brazil
Número total de Afiliações: 9
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 14, n. 9 SEP 12 2019.
Citações Web of Science: 1
Resumo

Methylation patterns established and maintained at CpG sites may be altered by single nucleotide polymorphisms (SNPs) within these sites and may affect the regulation of nearby genes. Our aims were to: 1) identify and generate a database of SNPs potentially subject to epigenetic control by DNA methylation via their involvement in creating, removing or displacing CpG sites (meSNPs), and; 2) investigate the association of these meSNPs with CpG islands (CGIs), and with methylation profiles of DNA extracted from tissues from cattle with divergent feed efficiencies detected using MIRA-Seq. Using the variant annotation for 56,969,697 SNPs identified in Run5 of the 1000 Bull Genomes Project and the UMD3.1.1 bovine reference genome sequence assembly, we identified and classified 12,836,763 meSNPs according to the nature of variation created at CpGs. The majority of the meSNPs were located in intergenic regions (68%) or introns (26.3%). We found an enrichment (p< 0.01) of meSNPs located in CGIs relative to the genome as a whole, and also in differentially methylated sequences in tissues from animals divergent for feed efficiency. Seven meSNPs, located in differentially methylated regions, were fixed for methylation site creating (MSC) or destroying (MSD) alleles in the differentially methylated genomic sequences of animals differing in feed efficiency. These meSNPs may be mechanistically responsible for creating or deleting methylation targets responsible for the differential expression of genes underlying differences in feed efficiency. Our methyl SNP database (dbmeSNP) is useful for identifying potentially functional ``epigenetic polymorphisms{''} underlying variation in bovine phenotypes. (AU)

Processo FAPESP: 15/20557-5 - Identificação de SNPs em sítios CpG localizados em regiões genômicas relacionadas à produção em bovinos
Beneficiário:Mariângela Bueno Cordeiro Maldonado
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 16/07584-6 - Efeitos fenotípicos de mutações em regiões reguladoras do genoma em bovinos
Beneficiário:Mariângela Bueno Cordeiro Maldonado
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado