Applied use of interferon-tau stimulated genes expression in polymorphonuclear cells to detect pregnancy compared to other early predictors in beef cattle

Autor(es)

de Melo, Gabriela Dalmaso ^[1] ; Mello, Barbara Piffero ^[1] ; Ferreira, Catia Aparecida ^[1] ; Souto Godoy Filho, Carlos Alberto ^[1] ; Rocha, Cecilia Constantino ^[1] ; Silva, Amanda Guimaraes ^[1] ; Reese, Sydney Taylor ^[2] ; Madureira, Ed Hoffmann ^[1] ; Pohler, Ky Garrett ^[2] ; Pugliesi, Guilherme ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Pirassununga, SP - Brazil

^[2] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 - USA

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 152, p. 94-105, AUG 2020.
• Citações Web of Science: 0

Resumo

We aimed to evaluate the accuracy of Interferon-tau stimulated genes (ISG) abundance in peripheral blood polymorphonuclear cells (PMNs) on D20 after fixed-time artificial insemination (FTAI; D0) as a pregnancy diagnosis method against CL evaluation by Doppler ultrasonography and progesterone (P4) concentrations on D20, as well as Pregnancy Associated Glycoproteins (PAG) concentrations on D25. Additionally, we evaluated the potential of ISG abundance in PMNs as pregnancy loss predictors. Nelore heifers (n = 103) and cows (n = 144) underwent estrous synchronization and were artificially inseminated on D0. Pregnancy was diagnosed by B-mode ultrasonography on D30 and D70, and after the final diagnosis, females were classified in four groups: Pregnant; Non-pregnant; Functional CL on D20 but non-pregnant (CL-NP) and Pregnancy loss between D30 and D70 (PL). After determining cutoff values, the Sensitivity (SE), Specificity (SP), Positive Predictive Value (PPV), Negative Predictive Value (NPV) and Accuracy (ACC) were determined for each method. All methods were classified as significant (P < 0.05) predictors of pregnancy. Both ISG expression and PAG concentrations were greater (P < 0.05) in pregnant females than in non-pregnant and CL-NP females but did not differ (P > 0.05) from the PL group. ISG15 expression was greater (P < 0.05) in heifers than in cows, but this difference was not found in OAS1 expreyyy (AU)

• Processo FAPESP: 17/13472-9 - Diagnóstico precoce da gestação em bovinos através da expressão de genes estimulados por interferon-tau em neutrófilos
• Beneficiário: Gabriela Dalmaso de Melo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/20108-4 - Uso de genes estimulados por interferon-tau e glicoproteínas associadas gestação para predizer a morte embrionária em vacas e novilhas Nelore
• Beneficiário: Gabriela Dalmaso de Melo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 15/10606-9 - Inovações no diagnóstico precoce da gestação em bovinos
• Beneficiário: Guilherme Pugliesi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores