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Evaluation of Phenolic Compound Toxicity Using a Bioluminescent Assay with the Fungus Gerronema viridilucens

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Autor(es):
Ventura, Fernanda F. [1, 2] ; Mendes, Luiz F. [1, 3] ; Oliveira, Anderson G. [4] ; Bazito, Reinaldo C. [1] ; Bechara, Etelvino J. H. [1] ; Freire, Renato S. [1] ; Stevani, V, Cassius
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] V, Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Sao Paulo, SP - Brazil
[2] Fundacao Jorge Duprat Figueiredo Seguranca & Med, Sao Paulo, SP - Brazil
[3] Bioat Grp, Santana Do Parnaiba, SP - Brazil
[4] Univ Sao Paulo, Inst Oceanog, Dept Oceanog Fis Quim & Geol, Sao Paulo, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Environmental Toxicology and Chemistry; JUN 2020.
Citações Web of Science: 0
Resumo

Basidiomycetes (phylum Basidiomycota) are filamentous fungi characterized by the exogenous formation of spores on a club-shaped cell called a basidium that are often formed on complex fruiting bodies (mushrooms). Many basidiomycetes serve an important role in recycling lignocellulosic material to higher trophic levels, and some show symbiotic relationships with plants. All known bioluminescent fungi are mushroom-forming basidiomycetes in the order Agaricales. Hence, the disruption of the basidiomycete community can entirely compromise the carbon cycle in nature from fungi to higher trophic levels. The fungus Gerronema viridilucens was used in the present study to investigate the toxicity of a phenolic compound series based on the inhibition of its bioluminescence. The median effect concentration (EC50) obtained from curves of bioluminescence inhibition versus log {[}phenolic compound] showed that 2,4,6-trichlorophenol was the most toxic compound in the series. The log EC50 values of all phenolic compounds were then used for the prediction of their toxicity. The univariate correlation of log EC50 values obtained from 6 different phenolic compounds was stronger with the dissociation constant (pK(a)) than with 1-octanol/water partition coefficient (K-OW). Nevertheless, the toxicity can be better predicted by using both parameters, suggesting that the phenol-driven uncoupling of fungus mitochondrial adenosine triphosphate synthesis is the origin of phenolic compound toxicity to the test fungus. Environ Toxicol Chem 2020;00:1-8.(c) 2020 SETAC (AU)

Processo FAPESP: 05/60484-5 - Desenvolvimento de bioensaio luminescente de toxicidade aguda com o fungo Gerronema viridilucens
Beneficiário:Luiz Fernando Mendes
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 17/22501-2 - Quimiexcitação eletrônica em sistemas biológicos: bioluminescência e 'foto'química no escuro
Beneficiário:Etelvino José Henriques Bechara
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/16885-1 - Bioluminescência em fungos: levantamento de espécies, estudo mecanístico & ensaios toxicológicos
Beneficiário:Cassius Vinicius Stevani
Linha de fomento: Auxílio à Pesquisa - Regular