Modulation of ERG Genes Expression in Clinical Isolates of Candida tropicalis Susceptible and Resistant to Fluconazole and Itraconazole

Autor(es)

Silva, Maria Claudia ^[1] ; Bonfim Carbone, Debora Cardozo ^[2] ; Diniz, Pedro Fernando ^[3] ; Fernandes, Fabricio Freitas ^[4] ; Fuzo, Carlos Alessandro ^[5] ; Pereira Cardoso Trindade, Carolina Santos ^[2] ; Chang, Marilene Rodrigues ^[3] ; Silva, Joao Santana ^{[1, 2, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto, SP - Brazil

^[2] Univ Catolica Dom Bosco, Postgrad Program Biotechnol, Campo Grande, MS - Brazil

^[3] Univ Fed Mato Grosso do Sul, Postgrad Program Infect & Parasit Dis, Campo Grande, MS - Brazil

^[4] Univ Sao Paulo, Preto Med Sch, Dept Cellular & Mol Biol, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Fiocruz Bi Inst Translat Med Plataform, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Mycopathologia; v. 185, n. 4 JUN 2020.
• Citações Web of Science: 0

Resumo

Candida tropicalis is a non-albicans Candida specie that causes candidosis in several countries, including Brazil. However, little is known about the mechanisms of drug resistance in C. tropicalis infections. In this study, we used clinical isolates of C. tropicalis susceptible as well as resistant to either Fluconazole or Itraconazole to assess the relationship between drug resistance and the expression of ERG and efflux pump genes. Our results showed that the main mechanism of resistance against both Fluconazole and Itraconazole in this specie is through the up-regulation of ERG rather than that of the efflux pump genes. We demonstrated that, although pre-treatment with azole drugs increases the expression of both ERG6 and ERG11 genes, the resistant or susceptible dose-dependent (SDD) samples are able to maintain high expression levels of these genes for longer periods of time than the susceptible samples. (AU)

• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs