The proteolytic fraction from Vasconcellea cundinamarcensis accelerates wound healing after corneal chemical burn in rabbits

Autor(es)

Silva, Rummenigge Oliveira ^[1] ; da Costa, Bruna Lopes ^[1] ; da Silva, Carolina Nunes ^[1] ; da Mata Martins, Thais Maria ^[2] ; Nunes Dourado, Lays Fernanda ^[1] ; de Goes, Alfredo Miranda ^[2] ; Lopes, Miriam Teresa ^[3] ; Salas, Carlos Edmundo ^[3] ; da Silva-Cunha, Armando ^[1] ; da Silva, Flavia Rodrigues ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Minas Gerais, Fac Farm, BR-31270901 Belo Horizonte, MG - Brazil

^[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Morfol, BR-31270901 Belo Horizonte, MG - Brazil

^[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BURNS; v. 46, n. 4, p. 928-936, JUN 2020.
• Citações Web of Science: 0

Resumo

Introduction: Chemical ocular burns are among the most frequently eye-related injuries, which require immediate and intensive evaluation and care since they may lead to potential complications such as superinfection, corneal perforation, and blindness. Vasconcellea cundinamarcensis, a species from Caricaceae family, contains highly active proteolytic enzymes in its latex that show healing activity in animal models bearing lesions of different etiologies. Methods: We evaluate the ocular toxicity of the proteolytic fraction from V. cundinamarcensis (P1G10) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Hen's Egg Test-Chorioallantoic Membrane test. The corneal healing property of P1G10 was studied by the ethanol-chemical burn in the rabbit's eyes. Results: P1G10 is safe for ocular administration, except when administrated at 10 mu g/mL. P1G10 at 1 mu g/mL accelerates the corneal re-epithelization achieving complete wound closure after 72h of chemical burn. Also, P1G10 modulated the inflammatory response and controlled the arrangement of collagen fibers in the stroma, demonstrating its potential corneal healing properties. Conclusions: Our work was the first one to evaluate the ophthalmic application of P1G10. Here we demonstrated that P1G10 is suitable for ocular administration and it has a promising corneal healing activity which may emerge as a new pharmacological tool to the development of a new drug for ocular surface chemical injuries in the future. (C) 2019 ISBI. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar
• Beneficiário: Maria Vitória Lopes Badra Bentley
• Modalidade de apoio: Auxílio à Pesquisa - Temático