Inhibitory Effect of a KSL-W Peptide-Loaded Poloxamer 407-Based Microemulsions for Buccal Delivery on Fusobacterium nucleatum Biofilm

Autor(es)

Bernegossi, Jessica ^[1] ; Fontana, Carla Raquel ^[2] ; Caiaffa, Karina Sampaio ^[3] ; Duque, Cristiane ^[4] ; Chorilli, Marlus ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Drugs & Med, Lab Pharmaceut Technol, Sch Pharmaceut Sci, Univ Estadual Paulista, BR-14800903 Araraquara, SP - Brazil

^[2] Sao Paulo State Univ, Dept Clin Anal, Lab Clin Microbiol, Sch Pharmaceut Sci, Univ Estadual Paulista, BR-14800903 Araraquara, SP - Brazil

^[3] Sao Paulo State Univ, Aracatuba Dent Sch, Dept Prevent & Restorat Dent, Univ Estadual Paulista, BR-16015050 Sao Paulo - Brazil

^[4] Sao Paulo State Univ, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, Univ Estadual Paulista, BR-16015050 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY; v. 16, n. 3, p. 390-397, MAR 2020.
• Citações Web of Science: 0

Resumo

KSL-W peptide has demonstrated antibacterial and antifungal activity and inhibitory effects against oral biofilm. This study aimed to check out the effect of chlorhexidine (CLX) or KSL-W peptide-loaded poloxamer 407-based microemulsions for buccal delivery on Fusobacterium nucleatum (F. nucleatum) biofilm. The formulation (F) containing 10% copolymer poloxamer 407 dispersion (1%), 40% oleic acid and 50% PPG-5-CETETH-20 was characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology, bioadhesive and syringeability; and in the treatment of a biofilm produced by F. nucleatum. The darkfield images obtained by PLM and the SAXS curves with an extended peak indicated that the system was characteristic of microemulsions. In a continuous analysis, microemulsions exhibited Newtonian behavior. In frequency, the oscillatory analysis profile presented predominantly viscous behavior. Bioadhesive force detected in the analysis of F (7.4 +/- 1.81 mN.s) and syringeability (17.83 +/- 5.97 N.mm) being adequate values for buccal administration. After 4 h, KSL-W-loaded F shown over 20% higher effectiveness than chlorhexidine-loaded microemulsions. In conclusion, the KSL-W-loaded microemulsions showed a considerable reduction in F. nucleatum biofilm formation and presented promising structural properties for buccal drug delivery. (AU)

• Processo FAPESP: 14/22045-9 - Sistemas precursores de cristais líquidos mucoadesivos para administração bucal de curcumina associados à terapia fotodinâmica no tratamento da candidose bucal
• Beneficiário: Jéssica Bernegossi
• Modalidade de apoio: Bolsas no Brasil - Doutorado