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Cytogenetic mechanisms of unisexuality in rock lizards

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Autor(es):
Spangenberg, Victor [1] ; Arakelyan, Marine [2] ; Cioffi, Marcelo de Bello [3] ; Liehr, Thomas [4] ; Al-Rikabi, Ahmed [4] ; Martynova, Elena [5] ; Danielyan, Felix [2] ; Stepanyan, Ilona [6] ; Galoyan, Eduard [7] ; Kolomiets, Oxana [1, 8]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] RAS, Vavilov Inst Gen Genet, Moscow - Russia
[2] Yerevan State Univ, Dept Zool, Yerevan - Armenia
[3] Univ Fed Sao Carlos, Dept Genet & Evolucao, LAb Citogenet Peixes, Sao Carlos, SP - Brazil
[4] Friedrich Schiller Univ, Jena Univ Hosp, Inst Human Genet, Jena - Germany
[5] Skolkovo Inst Sci & Technol, Moscow - Russia
[6] Sci Ctr Zool & Hydroecol, Yerevan - Armenia
[7] RAS, Severtsov Inst Ecol & Evolut, Moscow - Russia
[8] Galoyan, Eduard, RAS, Severtsov Inst Ecol \& Evolut, Moscow, Russia.Spangenberg, Victor, RAS, Vavilov Inst Gen Genet, Moscow - Russia
Número total de Afiliações: 8
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 MAY 26 2020.
Citações Web of Science: 0
Resumo

Darevskia rock lizards is a unique complex taxa, including more than thirty species, seven of which are parthenogenetic. In mixed populations of Darevskia lizards, tri- and tetraploid forms can be found. The most important issues in the theory of reticulate evolution of Darevskia lizards are the origin of parthenogenetic species and their taxonomic position. However, there is little data on how meiosis proceeds in these species. The present work reports the complex results of cytogenetics in a diploid parthenogenetic species - D. unisexualis. Here we detail the meiotic prophase I progression and the specific features.f mitotic chromosomes organization. The stages of meiosis prophase I were investigated by immunocytochemical analysis of preparations obtained from isolated primary oocytes of D. unisexualis in comparison with maternal species D. raddei nairensis. It has been shown that in D. unisexualis at the leptotene-zygotene stages the axial elements and the synaptonemal complex (SC) form typical ``bouquets{''}. At the pachytene-diplotene stage, 18 autosomal SC-bivalents and thickened asynapted sex Z and w univalents were observed. The presence of SYCP1 protein between the lateral elements of autosomal chromosomes proved the formation of assembled SCs. Comparative genomic hybridization (CGH) on the mitotic metaphase chromosomes of D. unisexualis was carried out using the genomic DNA isolated from the parental species D. raddei nairensis and D. valentini. In the pericentromeric regions of half of the mitotic chromosomes of D. unisexualis, specific regions inherited from maternal species have been found. Following our results, we suggest a model for diploid germ cells formation from diploid oocytes without premeiotic duplication of chromosomes in the oogenesis of diploid parthenogenetic lizards D. unisexualis. Taken as a whole, our findings confirm the hybrid nature of D. unisexualis and shed light on heterozygosity and automixis in diploid parthenogenetic forms. (AU)

Processo FAPESP: 18/22033-1 - Uma abordagem intercontinental para a investigação da evolução cromossômica, diversidade genética e biogeografia na ordem Osteoglossiformes (Teleostei: Osteoglossomorpha). Parte III.
Beneficiário:Marcelo de Bello Cioffi
Linha de fomento: Auxílio à Pesquisa - Regular