Proteomic profile of the saliva and plasma protein layer adsorbed on Ti-Zr alloy: the effect of sandblasted and acid-etched surface treatment

Autor(es)

Souza, Joao Gabriel Silva ^[1] ; Bertolini, Martinna ^[2] ; Costa, Raphael Cavalcante ^{[1, 3]} ; Lima, Carolina Veloso ^[4] ; Barao, Valentim Adelino Ricardo ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Piracicaba, SP - Brazil

^[2] Univ Connecticut UCONN, Sch Dent Med, Farmington, CT - USA

^[3] Lima, Carolina Veloso, UNINASSAU, Dent Sch, Teresina, PI, Brazil.Souza, Joao Gabriel Silva, Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Piracicaba, SP - Brazil

^[4] UNINASSAU, Dent Sch, Teresina, PI - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 36, n. 4 MAY 2020.
• Citações Web of Science: 0

Resumo

Titanium-zirconium (Ti-Zr) alloy has been widely used as a biomaterial for implant devices, and it is commonly treated by sandblasting followed by acid etching (SLA) to improve biological responses. Although protein adsorption is the first biological response, the effect of this SLA treatment on the proteomic profile of proteins adsorbed from saliva and blood plasma has not been tested. In this study, the proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).Streptococcus sanguiniswas used to test whether the protein layer affects bacterial adhesion. SLA treatment affected the proteomic profile, showing exclusive proteins adsorbed from saliva (14) and plasma (3). However, both groups exhibited close patterns of intensity for common proteins, molecular functions and biological processes mediated by proteins. Interestingly, Ti-Zr(SLA)showed higher bacterial adhesion (similar to 1.9 fold over) for the surface coated with plasma proteins. Therefore, SLA treatment of Ti-Zr alloy changed the proteomic profile, which may affect bacterial adhesion. (AU)

• Processo FAPESP: 16/11470-6 - Deposição por pulverização catódica de filmes de óxido de tântalo (Ta2O5) na superfície de titânio para aplicações biomédicas: comportamento eletroquímico, biocompatibilidade e análise microbiológica
• Beneficiário: Valentim Adelino Ricardo Barão
• Modalidade de apoio: Auxílio à Pesquisa - Regular