Radioadapted Wangiella dermatitidis senses radiation in its environment in a melanin-dependent fashion

Autor(es)

Malo, Mackenzie E. ^[1] ; Frank, Connor ^{[1, 2]} ; Dadachova, Ekaterina ^[2]

Afiliação do(s) autor(es)

^[1] Univ Saskatchewan, Coll Pharm & Nutr, Saskatoon, SK S7N 5E5 - Canada

^[2] Dadachova, Ekaterina, Univ Saskatchewan, Coll Pharm \& Nutr, Saskatoon, SK S7N 5E5, Canada.Malo, Mackenzie E., Univ Saskatchewan, Coll Pharm & Nutr, Saskatoon, SK S7N 5E5 - Canada

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 124, n. 5, p. 368-375, MAY 2020.
• Citações Web of Science: 1

Resumo

Black fungi withstand extreme stresses partly due to the presence of melanin. Melanin is associated with structural integrity and resistance to chemical and radiation stress. This results in improved health and fitness, specifically in extreme conditions. Our goal was to exploit the radiation sensing nature of melanized fungus in order to develop a radioadapted strain capable of responding to radiation in the environment. The protracted exposure of a melanized fungus, Wangiella dermatitidis, to a mixed source of radiation altered the electron transport properties. There was no effect in an albino mutant wdpsk1. We then tested the growth response to radiation in the environment, with shielding from direct exposure to the radiation. Gamma radiation caused increased colony growth irrespective of exposure history in melanized fungus. Beta particles produced growth inhibition. The previously exposed melanized strain demonstrated colony growth in response to alpha particles in the environment. Alpha particles have a higher linear energy transfer, which produces more reactive oxygen species. Our previously exposed melanized strain was resistant to the toxic effects of H2O2, while the naive and non-melanized strains were sensitive. We propose that previous radiation exposure introduces adaptations that equip melanized fungi to tolerate, sense, and respond to radiation byproducts. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 18/20571-6 - International Symposium on Fungal Stress - ISFUS
• Beneficiário: Drauzio Eduardo Naretto Rangel
• Modalidade de apoio: Auxílio Organização - Reunião Científica