Canine Visceral Leishmaniasis in Sao Paulo, Brazil, the Most Populous City of South America: Isolation, Molecular Diagnosis, and Phylogenetic Inferences

Autor(es)

Marcili, Arlei ^{[1, 2]} ; da Silva, Ryan Emiliano ^[2] ; da Costa, Vilma Pereira ^{[1, 3]} ; Nieri-Bastos, Fernanda A. ^[2] ; de Freitas e Azevedo, Roberta Carvalho ^{[1, 3]} ; Moraes Filho, Jonas ^{[1, 3]} ; Tonhosolo, Renata ^[4]

Afiliação do(s) autor(es)

^[1] Univ Santo Amaro, Dept Med & Anim Welf, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Vet Med & Anim Hlth, Sao Paulo - Brazil

^[3] Tonhosolo, Renata, Univ Santo Amaro, Med Coll, Sao Paulo, Brazil.Marcili, Arlei, Univ Santo Amaro, Dept Med & Anim Welf, Sao Paulo - Brazil

^[4] Univ Santo Amaro, Med Coll, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VECTOR-BORNE AND ZOONOTIC DISEASES; v. 20, n. 10 JUN 2020.
• Citações Web of Science: 0

Resumo

Background and Objectives:Canine visceral leishmaniasis affects dogs, the main urban reservoirs, which favor the transmission and expansion of this zoonotic disease in areas with high anthropization process and human density. We investigated the occurence ofLeishmania infatumbased in molecular diagnosis, and phylogenetic analysis of isolates obtained from dogs in metropolitan region of Sao Paulo. Methods:A total of 201 dogs were tested by parasitological and molecular diagnosis. Phylogenetic analysis based sequences from SSUrDNA and gGAPDH genes were performed. Results:The parasitological diagnosis revealed 5% (10/201) of positivity, and the sequences obtained from seven isolates were clustered with L.infantumin phylogentic analysis based on SSUrDNA and gGAPDH genes. A total of 24.9% (50/201) of dogs were positive in molecular diagnosis based on cathepsin L-likemarker. Interpretation and Conclusion:According to this study, it is necessary to implement a surveillance policy of visceral leishmaniasis, intensifying the actions of diagnosis, prevention, and control of this zoonosis. (AU)

• Processo FAPESP: 15/25592-3 - Taxonomia e filogenia do gênero Trypanosoma: descrição de novas espécies que infectam animais silvestres
• Beneficiário: Arlei Marcili
• Modalidade de apoio: Auxílio à Pesquisa - Regular