Wettability and pre-osteoblastic behavior evaluations of a dense bovine hydroxyapatite ceramics

Autor(es)

Pires, Luara A. ^[1] ; de Meira, Camila R. ^[2] ; Tokuhara, Cintia K. ^[3] ; de Oliveira, Flavia A. ^[3] ; Dainezi, Vanessa B. ^[4] ; Graeff, Marcia S. Zardin ^[3] ; Fortulan, Carlos A. ^[2] ; de Oliveira, Rodrigo C. ^[3] ; Puppin-Rontani, Regina M. ^[4] ; Borges, Ana Flavia S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Ave Doctor Otavio Pinheiro Brisola 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mech Engn, Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[4] Univ Estadual Campinas, Dept Pediat Dent, Fac Dent Piracicaba, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL SCIENCE; v. 62, n. 3, p. 259-264, JUL 2020.
• Citações Web of Science: 0

Resumo

In this study, the wettability, cell viability, and roughness of an experimental dense bovine hydroxyapatite {[}Ca-10(PO4)(6)(OH)(2)] ceramic block were evaluated so that, in the future, it could be used as a base material for dental implants. The results to commercial zirconia and a commercially pure titanium (Ti) alloy were compared. The surface roughness and contact angles were measured. An in vitro evaluation was conducted by means of tests in which pre-osteoblastic MC3T3-E1 cells were placed in indirect and direct contact with these materials. For cell viability, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and crystal violet test were conducted. A qualitative analysis was conducted using variable pressure scanning electron microscopy (SEM). No statistically significant differences were observed in wettability and roughness tests among the groups. In both the MTT assay and crystal violet test, all groups demonstrated satisfactory results without cytotoxicity. SEM showed cell adhesion and cell proliferation results on the material surfaces after 24 h and 48 h. In conclusion, this dense Ca-10(PO4)(6)(OH)(2) ceramic can be considered as a potential biocompatible material. (AU)

• Processo FAPESP: 11/18061-0 - Análise mecânica, estrutural e de copings de zircônia experimental para uso em sistema CAD/CAM
• Beneficiário: Ana Flávia Sanches Borges
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/07283-5 - Avaliação in vitro da citotoxicidade celular de zircônia experimental frente a células osteoblásticas
• Beneficiário: Marianne Barbosa Salgado de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/06738-9 - Avaliação de materiais, instrumentos e protocolos de irrigação empregados no tratamento endodôntico, por meio da microscopia eletrônica de varredura e micro análise de raios X.
• Beneficiário: Marco Antonio Hungaro Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/23639-0 - Manufatura de cerâmica densa de hidroxiapatita bovina com adição de nanopartículas de TiO2: inovação para sustentabilidade
• Beneficiário: Ana Flávia Sanches Borges
• Modalidade de apoio: Auxílio à Pesquisa - Regular