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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Wettability and pre-osteoblastic behavior evaluations of a dense bovine hydroxyapatite ceramics

Full text
Author(s):
Pires, Luara A. [1] ; de Meira, Camila R. [2] ; Tokuhara, Cintia K. [3] ; de Oliveira, Flavia A. [3] ; Dainezi, Vanessa B. [4] ; Graeff, Marcia S. Zardin [3] ; Fortulan, Carlos A. [2] ; de Oliveira, Rodrigo C. [3] ; Puppin-Rontani, Regina M. [4] ; Borges, Ana Flavia S. [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Ave Doctor Otavio Pinheiro Brisola 9-75, BR-17012901 Bauru, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mech Engn, Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[4] Univ Estadual Campinas, Dept Pediat Dent, Fac Dent Piracicaba, Piracicaba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF ORAL SCIENCE; v. 62, n. 3, p. 259-264, JUL 2020.
Web of Science Citations: 0
Abstract

In this study, the wettability, cell viability, and roughness of an experimental dense bovine hydroxyapatite {[}Ca-10(PO4)(6)(OH)(2)] ceramic block were evaluated so that, in the future, it could be used as a base material for dental implants. The results to commercial zirconia and a commercially pure titanium (Ti) alloy were compared. The surface roughness and contact angles were measured. An in vitro evaluation was conducted by means of tests in which pre-osteoblastic MC3T3-E1 cells were placed in indirect and direct contact with these materials. For cell viability, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and crystal violet test were conducted. A qualitative analysis was conducted using variable pressure scanning electron microscopy (SEM). No statistically significant differences were observed in wettability and roughness tests among the groups. In both the MTT assay and crystal violet test, all groups demonstrated satisfactory results without cytotoxicity. SEM showed cell adhesion and cell proliferation results on the material surfaces after 24 h and 48 h. In conclusion, this dense Ca-10(PO4)(6)(OH)(2) ceramic can be considered as a potential biocompatible material. (AU)

FAPESP's process: 11/18061-0 - Structural and mechanical analysis and copings of experimental zirconia for use CAD/CAM system
Grantee:Ana Flávia Sanches Borges
Support Opportunities: Regular Research Grants
FAPESP's process: 12/07283-5 - In vitro citotoxicity evaluation of experimental zirconia against osteoblasts cells
Grantee:Marianne Barbosa Salgado de Oliveira
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/06738-9 - Evaluation of materials, instruments and protocols of irrigation used in Endodontic treatment, by means of scanning electron microscopy and micro analysis of x-ray.
Grantee:Marco Antonio Hungaro Duarte
Support Opportunities: Regular Research Grants
FAPESP's process: 18/23639-0 - Manufacturing of dense bovine hydroxyapatite ceramics with added TiO2 nanoparticles: innovation for sustainability
Grantee:Ana Flávia Sanches Borges
Support Opportunities: Regular Research Grants