Replication origin location might contribute to genetic variability in Trypanosoma cruzi

Autor(es):Mostrar menos -

de Araujo, Christiane Bezerra ^{[1, 2]} ; Chagas da Cunha, Julia Pinheiro ^{[1, 2]} ; Inada, Davi Toshio ^{[1, 2]} ; Damasceno, Jeziel ^[3] ; Jeronimo Lima, Alex Ranieri ^[4] ; Hiraiwa, Priscila ^[5] ; Marques, Catarina ^[3] ; Goncalves, Evonnildo ^[4] ; Nishiyama-Junior, Milton Yutaka ^{[6, 1]} ; McCulloch, Richard ^[3] ; Elias, Maria Carolina ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo - Brazil

^[2] Inst Butantan, Lab Ciclo Celular, Sao Paulo - Brazil

^[3] Univ Glasgow, Inst Infect Immun & Inflammat, Wellcome Ctr Mol Parasitol, Glasgow, Lanark - Scotland

^[4] Univ Fed Para, Lab Tecnol Biomol Bioinformat, Inst Ciencias Biol, Belem, Para - Brazil

^[5] Inst Carlos Chagas, Fundacao Oswaldo Cruz, Curitiba, Parana - Brazil

^[6] Inst Butantan, Lab Especial Toxinol Aplicada, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Genomics; v. 21, n. 1 JUN 22 2020.
• Citações Web of Science: 0

Resumo

BackgroundDNA replication in trypanosomatids operates in a uniquely challenging environment, since most of their genomes are constitutively transcribed. Trypanosoma cruzi, the etiological agent of Chagas disease, presents high variability in both chromosomes size and copy number among strains, though the underlying mechanisms are unknown.ResultsHere we have mapped sites of DNA replication initiation across the T. cruzi genome using Marker Frequency Analysis, which has previously only been deployed in two related trypanosomatids. The putative origins identified in T. cruzi show a notable enrichment of GC content, a preferential position at subtelomeric regions, coinciding with genes transcribed towards the telomeres, and a pronounced enrichment within coding DNA sequences, most notably in genes from the Dispersed Gene Family 1 (DGF-1).ConclusionsThese findings suggest a scenario where collisions between DNA replication and transcription are frequent, leading to increased genetic variability, as seen by the increase SNP levels at chromosome subtelomeres and in DGF-1 genes containing putative origins. (AU)

• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/24170-5 - Dinâmica da replicação do DNA em Trypanosoma cruzi: caracterização do licenciamento e da taxa de replicação
• Beneficiário: Marcelo Santos da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites?
• Beneficiário: Maria Carolina Quartim Barbosa Elias Sabbaga
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/13375-5 - Origens da replicação em tripanossomas
• Beneficiário: Christiane Bezerra de Araujo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado