Bactericidal Effect of Entomopathogenic BacteriumPseudomonas entomophilaAgainstXanthomonas citriReduces Citrus Canker Disease Severity

Autor(es)

Villamizar, Sonia ^[1] ; Ferro, Jesus Aparecido ^[1] ; Caicedo, Juan Carlos ^{[1, 2]} ; Alves, L. M. C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, Post Grad Program Agr & Livestock Microbiol, Jaboticabal - Brazil

^[2] Univ Santander UDES, Res Grp CIBAS, Fac Exact Nat & Agr Sci, Bucaramanga - Colombia

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 11, JUN 24 2020.
• Citações Web of Science: 0

Resumo

The bacteriumPseudomonas entomophilahas been recognized as an exceptional species within thePseudomonasgenus, capable of naturally infecting and killing insects from at least three different orders.P. entomophilaingestion leads to irreversible gut damage resulting from a global blockage of translation, which impairs both immune and tissue repair systems in the insect intestine. In this study we isolated aP. entomophilabacterial strain from soil samples which displayed a strong activity againstXanthomonas citrisubsp,citri(Xcc), the etiological agent of citrus canker disease. The antagonism potential of isolated bacteria againstXccand its ability to reduce citrus canker severity was assessed bothex plantaandin planta. Our findings show that pathogenicity assays inCitrus x limoniaby pressure infiltration and spray with a mixture ofP. entomophilaandXccleaded to a significant reduction in the number of canker lesions in high susceptible citrus leaves, at 21 days post-infection. To the best of our knowledge this is the first report of antibacterial activity ofP. entomophilaagainst a phytopathogenic bacterium. Collective action ofP. entomophilafactors such as diketopiperazine production and the type 6 secretion system (T6SS) may be involved in this type of biological control of citrus canker. The results suggest that theP. entomophilastrain could be a promising biocontrol agent acting directly againstXcc. (AU)

• Processo FAPESP: 15/22473-3 - IDENTIFICAÇÃO DE SENSORE(S) ALTERNATIVO(S) PARA O AUTOINDUTOR DSF, REGULADOR DA PATOGENICIDADE EM Xanthomonas citri subsp. citri
• Beneficiário: Juan Carlos Caicedo Cepeda
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado