Placental transcriptome profile of women with sickle cell disease reveals differentially expressed genes involved in migration, trophoblast differentiation and inflammation

Autor(es)

Baptista, Leticia Carvalho ^[1] ; Costa, Maria Laura ^[2] ; Surita, Fernanda Garanhani ^[2] ; Rocha, Cristiane de Souza ^[3] ; Lopes-Cendes, Iscia ^[3] ; de Souza, Bruno Batista ^[1] ; Costa, Fernando Ferreira ^[4] ; de Melo, Monica Barbosa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Ctr Mol Biol & Genet Engn CBMEG, BR-13083875 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Obstet & Gynecol, BR-13083880 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Med Sci, Dept Med Genet & Genom Med, BR-13083887 Campinas, SP - Brazil

^[4] Univ Campinas UNICAMP, Hematol & Hemotherapy Ctr, BR-13083878 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BLOOD CELLS MOLECULES AND DISEASES; v. 84, SEP 2020.
• Citações Web of Science: 0

Resumo

Sickle cell disease (SCD) is a group of disorders whose common characteristic is the presence of hemoglobin (Hb) S in erythrocytes. The main consequence of this abnormality is vaso-occlusion, which can affect almost all organs including the placenta. This study aimed to evaluate the gene expression profile in placentas of women with SCD by means of total RNA sequencing. For this, we proposed a case-control study, with three groups of pregnant women: HbSS (n = 10), HbSC (n = 14) and HbAA (n = 21). The results showed differences in expression in a number of genes such as NOS2 (fold change, FC = 4.52), HLAG (FC = 5.56), ASCL2 (FC = 3.61), CXCL10 (FC = -3.66) and IL1R2 (FC = 3.92) for the HbSC group and S100A8 (FC = -3.82), CPXM2 (FC = 4.57), CXCL10 (FC = -4.59), CXCL11 (FC = -3.72) and CAMP (FC = -4.55) for the HbSS group. Differentially expressed genes are mainly associated with migration, trophoblast differentiation and inflammation. The causes leading to altered gene expression in placentas of sickle cell patients are not fully understood, but the presence of intravascular hemolysis and vaso-occlusion, with cycles of ischemia and reperfusion, may contribute to the emergence of an environment which can be very harmful for placental physiology, altering the nutrient supply and metabolic exchange for fetal growth. (AU)

• Processo FAPESP: 15/08330-5 - Transcriptômica em placentas de pacientes portadoras de doença falciforme
• Beneficiário: Letícia de Carvalho Baptista
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/00984-3 - Doenças dos glóbulos vermelhos: fisiopatologia e novas abordagens terapêuticas
• Beneficiário: Fernando Ferreira Costa
• Modalidade de apoio: Auxílio à Pesquisa - Temático