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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17 alpha-ethynylestradiol on membrane models of DPPC/cholesterol

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Autor(es):
Marques Ruiz, Gilia Cristine [1] ; Pazin, Wallance Moreira [1] ; do Carmo Morato, Luis Fernando [1] ; Oliveira Jr, Osvaldo N. ; Leopoldo Constantino, Carlos Jose [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Technol & Appl Sci, Dept Phys, Presidente Prudente, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF MOLECULAR LIQUIDS; v. 311, AUG 1 2020.
Citações Web of Science: 0
Resumo

Risks to human health have been reported owing to prolonged exposure to hormones, whose action depends on their molecular-level interaction with cell membranes. In this study, we investigate the interaction of the synthetic hormone 17 a-ethynylestradiol (EE2) in two different membrane models, Langmuir monolayers and giant unilamellar vesicles (GUVs) made with a binary mixture of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol (Chol) in order to mimic the plasma membrane of mammalian cells. EE2 was found to expand the Langmuir monolayers, with shifts to larger areas per molecule in the surface pressure isotherm. In all of these observations, stronger effects were noted for the DPPC/Chol monolayers with X-Chol = 0.3, which mimics the proportion of phospholipid/sterol in the plasma membrane. At high surface pressures, EE2 is believed to weaken the attractive interactions between DPPC and Chol, in addition to affecting the ordering of the lipid chains as indicated in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) measurements. In GUVs obtained with X-Chol = 0.3 mixtures, EE2 induced a phase contrast loss as a result of increased permeability of the lipid bilayer. The results with Langmuir monolayers and GUV combined point to EE2 action on representative cell membranes, which can be correlated with physiological effects caused by indirect intake of EE2. (C) 2020 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
Beneficiário:Osvaldo Novais de Oliveira Junior
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/09633-4 - Mecanismo de ação do composto bioativo Artepillin C em sistemas miméticos de membranas e membranas citoplasmáticas de células tumorais
Beneficiário:Wallance Moreira Pazin
Linha de fomento: Bolsas no Brasil - Pós-Doutorado