Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells

Autor(es)

Coaguila-Llerena, H. ^[1] ; Rodrigues, E. M. ^[1] ; Santos, C. S. ^[1] ; Ramos, S. G. ^[2] ; Medeiros, M. C. ^[3] ; Chavez-Andrade, G. M. ^[1] ; Guerreiro-Tanomaru, J. M. ^[1] ; Tanomaru-Filho, M. ^[1] ; Faria, G. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Restorat Dent, Araraquara Sch Dent, Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol, Ribeirao Preto - Brazil

^[3] Sao Paulo State Univ UNESP, Araraquara Sch Dent, Dept Oral Diag & Surg, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 53, n. 9 JUL 2020.
• Citações Web of Science: 0

Resumo

Aim To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT mixed with sodium hypochlorite - NaOCl (NaOCl/OCT). Methodology L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and alpha-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (alpha = 0.05). Results MTT and NR assays revealed that 0.1% OCT had the least cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the greatest cytotoxicity (P < 0.05). The OCT group had a greatest percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions did not induce ALP activity. Conclusion Octenidine dihydrochloride was less cytotoxic, induced apoptosis at higher doses, caused few changes in the cytoskeleton and did not induce alkaline phosphatase activity. In addition, octenidine dihydrochloride reduced the cytotoxicity of 5.25% NaOCl when combined at 20 and 50%. (AU)

• Processo FAPESP: 18/24662-6 - Propriedades físico-químicas, citotoxicidade e penetrabilidade nos túbulos dentinários do hipoclorito de cálcio com ou sem surfactantes
• Beneficiário: Eric Hernán Coaguila Llerena
• Modalidade de apoio: Bolsas no Brasil - Doutorado