Type I interferon receptors and interferon-tau-stimulated genes in peripheral blood mononuclear cells and polymorphonuclear leucocytes during early pregnancy in beef heifers

Autor(es)

Melo, G. D. ^[1] ; Pinto, L. M. F. ^[1] ; Rocha, C. C. ^[1] ; Motta, I. G. ^[1] ; Silva, L. A. ^[2] ; da Silveira, J. C. ^[2] ; Gonella-Diaza, A. M. ^[3] ; Binelli, M. ^[4] ; Pugliesi, G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Ave Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Vet Med, Ave Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[3] Univ Florida, Inst Food & Agr Sci, North Florida Res & Educ Ctr, 4932 Highway 162, Marianna, FL 32446 - USA

^[4] Univ Florida, Dept Anim Sci, 2250 Shealy Dr, Gainesville, FL 32608 - USA

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 32, n. 11, p. 953-966, 2020.
• Citações Web of Science: 1

Resumo

This study characterised the expression of interferon (IFN)-tau-stimulatcd genes (ISGs) and Type I IFN receptors in circulating polymorphonuclear cells (PMNs) of beef heifers and compared it with expression in peripheral blood mononuclear cells (PBMCs) up to Day 20 of gestation. Nelore heifers (n = 26) were subjected to fixed-time AI (FTAI) on Day 0. PMNs and PBMCs were isolated on Days 0, 10, 14, 16, 18 and 20 after FTAI. The abundance of target transcripts (ubiquitin-like protein (ISG15), 2'-5'-oligoadenylate synthetase 1 (OAS1), myxovirus resistance 1 (MX1), myxovirus resistance 2 (MX2), IFN receptor 1 (IFNAR1) and IFN receptor 2 (IFNAR2)) was determined using real-time quantitative polymerase chain reaction and compared between pregnant (n = 8) and non-pregnant (n = 9) females. In both PBMCs and PMNs, ISG15 and OAST expression was greater in pregnant than non-pregnant heifers on Days 18 and 20. There were no significant differences in the expression of ISGs between PBMCs and PMNs. A time effect on expression was found for IFNAR1 in PBMCs and IFNAR2 in PMNs, with decreased expression of both genes on Days 18 and 20. When the expression of these genes was compared between cell types only in pregnant heifers, IFNAR2 expression in PMNs had an earlier decrease when compared to its expression in PBMCs, starting from Day 18. In conclusion, PMNs do not respond earlier to the conceptus stimulus, and ISG15 and OAST expression in both PMNs and PBMCs can be used as a suitable marker for pregnancy diagnosis on Days 18 and 20. In addition, gestational status did not affect IFNAR1 and IFNAR2 expression, but IFNAR2 showed a distinct response between PMNs and PBMCs of pregnant heifers. (AU)

• Processo FAPESP: 17/13472-9 - Diagnóstico precoce da gestação em bovinos através da expressão de genes estimulados por interferon-tau em neutrófilos
• Beneficiário: Gabriela Dalmaso de Melo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/10606-9 - Inovações no diagnóstico precoce da gestação em bovinos
• Beneficiário: Guilherme Pugliesi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores