Multiplatform Investigation of Plasma and Tissue Lipid Signatures of Breast Cancer Using Mass Spectrometry Tools

Autor(es):Mostrar menos -

Rosini Silva, Alex Ap. ^[1] ; Cardoso, Marcella R. ^[2] ; Rezende, Luciana Montes ^[2] ; Lin, John Q. ^[3] ; Guimaraes, Fernando ^[2] ; Paiva Silva, Geisilene R. ^[4] ; Murgu, Michael ^[5] ; Priolli, Denise Goncalves ^[1] ; Eberlin, Marcos N. ^[6] ; Tata, Alessandra ^[7] ; Eberlin, Livia S. ^[3] ; Derchain, Sophie F. M. ^[2] ; Porcari, Andreia M. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Francisco Univ, Postgrad Program Hlth Sci, BR-12916900 Braganca Paulista, SP - Brazil

^[2] State Univ Campinas UNICAMP, Fac Med Sci, Dept Gynecol & Breast Oncol, Womens Hosp CAISM, BR-13083881 Campinas, SP - Brazil

^[3] Univ Texas Austin, Dept Chem, Austin, TX 78712 - USA

^[4] State Univ Campinas UNICAMP, Fac Med Sci, Womens Hosp CAISM, Lab Mol & Investigat Pathol, BR-13083881 Campinas, SP - Brazil

^[5] Waters Corp, BR-13083970 Sao Paulo, SP - Brazil

^[6] Univ Prebiteriana Mackenzie, Sch Engn, BR-01302907 Sao Paulo, SP - Brazil

^[7] Ist Zooprofilatt Sperimentale Venezie, Lab Chim Sperimentale, Viale Fiume 78, I-36100 Vicenza - Italy

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 10 MAY 2020.
• Citações Web of Science: 0

Resumo

Plasma and tissue from breast cancer patients are valuable for diagnostic/prognostic purposes and are accessible by multiple mass spectrometry (MS) tools. Liquid chromatography-mass spectrometry (LC-MS) and ambient mass spectrometry imaging (MSI) were shown to be robust and reproducible technologies for breast cancer diagnosis. Here, we investigated whether there is a correspondence between lipid cancer features observed by desorption electrospray ionization (DESI)-MSI in tissue and those detected by LC-MS in plasma samples. The study included 28 tissues and 20 plasma samples from 24 women with ductal breast carcinomas of both special and no special type (NST) along with 22 plasma samples from healthy women. The comparison of plasma and tissue lipid signatures revealed that each one of the studied matrices (i.e., blood or tumor) has its own specific molecular signature and the full interposition of their discriminant ions is not possible. This comparison also revealed that the molecular indicators of tissue injury, characteristic of the breast cancer tissue profile obtained by DESI-MSI, do not persist as cancer discriminators in peripheral blood even though some of them could be found in plasma samples. (AU)

• Processo FAPESP: 16/07822-4 - Análise De Biomarcadores Moleculares Associados À Quimiorresistência Mediada Por Células Tronco Tumorais De Mama
• Beneficiário: Sophie Françoise Mauricette Derchain
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/04314-6 - Imageamento e metabolômica por espectrometria de massas aplicada ao estudo de resistência à terapia neoadjuvante para tratamento de câncer de mama: busca por um teste preditivo
• Beneficiário: Andréia de Melo Porcari
• Modalidade de apoio: Auxílio à Pesquisa - Regular