Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry

Autor(es)

Ribeiro, Ligia N. M. ^{[1, 2]} ; Franz-Montan, Michelle ^[3] ; Alcantara, Ana C. S. ^[4] ; Breitkreitz, Marcia C. ^[5] ; Castro, Simone R. ^[1] ; Guilherme, Viviane A. ^[1] ; Muniz, V, Bruno ; Rodrigues da Silva, Gustavo H. ^[1] ; de Paula, Eneida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Rua Monteiro Lobato 255, Cidade Univ, BR-13083862 Campinas, SP - Brazil

^[2] Univ Fed Uberlandia, Sch Vet Med, UFU, Uberlandia, MG - Brazil

^[3] V, Univ Estadual Campinas, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, SP - Brazil

^[4] Univ Fed Maranhao, Dept Chem, Sao Luis, Maranhao - Brazil

^[5] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, UNICAMP, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 JUL 9 2020.
• Citações Web of Science: 0

Resumo

Topical anesthetics are widely applied in order to relieve the discomfort and anxiety caused by needle insertion and other painful superficial interventions at the oral cavity. So far, there are no commercially available effective topical anesthetic formulations for that purpose, and the most of developments are related to hydrophilic and low mucoadhesive forms. Therefore, we have prepared different hybrid nanofilms composed of biopolymer matrices (chitosan, pectin, and chitosan-pectin) blended with nanostructured lipid carriers (NLC) loading the eutectic mixture of 5% lidocaine-prilocaine (LDC-PLC), in order to fulfill this gap in the market. These dual systems were processed as hybrid nanofilms by the solvent/casting method, and its mucoadhesive, structural and mechanical properties were detailed. The most appropriate hybrid nanofilm combined the advantages of both pectin (PCT) and NLC components. The resultant material presented sustained LDC-PLC release profile for more than 8 h; permeation across porcine buccal mucosa almost twice higher than control and non-cytotoxicity against 3T3 and HACAT cell lines. Then, the in vivo efficacy of PCT/NLC formulation was compared to biopolymer film and commercial drug, exhibiting the longest-lasting anesthetic effect (>7 h), assessed by tail flick test in mice. These pectin-based hybrid nanofilms open perspectives for clinical trials and applications beyond Dentistry. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/25372-0 - Desenvolvimento de carreadores lipídicos nanoestruturados para encapsulação de anestésicos locais processados em matrizes biopoliméricas para aplicação tópica em mucosa oral
• Beneficiário: Lígia Nunes de Morais Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/14457-5 - Carreadores baseados em lipídios (SLN/NLC e lipossomas com gradiente iônico) como estratégia para aumentar a encapsulação e a potência de anestésicos locais
• Beneficiário: Eneida de Paula
• Modalidade de apoio: Auxílio à Pesquisa - Temático