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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hybrid nanofilms as topical anesthetics for pain-free procedures in dentistry

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Author(s):
Ribeiro, Ligia N. M. [1, 2] ; Franz-Montan, Michelle [3] ; Alcantara, Ana C. S. [4] ; Breitkreitz, Marcia C. [5] ; Castro, Simone R. [1] ; Guilherme, Viviane A. [1] ; Muniz, V, Bruno ; Rodrigues da Silva, Gustavo H. [1] ; de Paula, Eneida [1]
Total Authors: 9
Affiliation:
[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Rua Monteiro Lobato 255, Cidade Univ, BR-13083862 Campinas, SP - Brazil
[2] Univ Fed Uberlandia, Sch Vet Med, UFU, Uberlandia, MG - Brazil
[3] V, Univ Estadual Campinas, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, SP - Brazil
[4] Univ Fed Maranhao, Dept Chem, Sao Luis, Maranhao - Brazil
[5] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, UNICAMP, Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 10, n. 1 JUL 9 2020.
Web of Science Citations: 0
Abstract

Topical anesthetics are widely applied in order to relieve the discomfort and anxiety caused by needle insertion and other painful superficial interventions at the oral cavity. So far, there are no commercially available effective topical anesthetic formulations for that purpose, and the most of developments are related to hydrophilic and low mucoadhesive forms. Therefore, we have prepared different hybrid nanofilms composed of biopolymer matrices (chitosan, pectin, and chitosan-pectin) blended with nanostructured lipid carriers (NLC) loading the eutectic mixture of 5% lidocaine-prilocaine (LDC-PLC), in order to fulfill this gap in the market. These dual systems were processed as hybrid nanofilms by the solvent/casting method, and its mucoadhesive, structural and mechanical properties were detailed. The most appropriate hybrid nanofilm combined the advantages of both pectin (PCT) and NLC components. The resultant material presented sustained LDC-PLC release profile for more than 8 h; permeation across porcine buccal mucosa almost twice higher than control and non-cytotoxicity against 3T3 and HACAT cell lines. Then, the in vivo efficacy of PCT/NLC formulation was compared to biopolymer film and commercial drug, exhibiting the longest-lasting anesthetic effect (>7 h), assessed by tail flick test in mice. These pectin-based hybrid nanofilms open perspectives for clinical trials and applications beyond Dentistry. (AU)

FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Marco Aurelio Zezzi Arruda
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/25372-0 - Development of nanostructured lipid carriers encapsulating local anesthetics processed biopolymeric matrices for topical application at oral mucosa
Grantee:Lígia Nunes de Morais Ribeiro
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics
Grantee:Eneida de Paula
Support Opportunities: Research Projects - Thematic Grants