Dynamic Expression of Long Non-Coding RNAs Throughout Parasite Sexual and Neural Maturation in Schistosoma Japonicum

Autor(es)

Maciel, Lucas F. ^{[1, 2]} ; Morales-Vicente, David A. ^{[3, 1]} ; Verjovski-Almeida, Sergio ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Expressao Gen Eucariotos, BR-05503900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Matemat & Estat, Programa Interunidades Bioinformat, BR-05508900 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NON-CODING RNA; v. 6, n. 2 JUN 2020.
• Citações Web of Science: 0

Resumo

Schistosoma japonicumis a flatworm that causes schistosomiasis, a neglected tropical disease. S. japonicumRNA-Seq analyses has been previously reported in the literature on females and males obtained during sexual maturation from 14 to 28 days post-infection in mouse, resulting in the identification of protein-coding genes and pathways, whose expression levels were related to sexual development. However, this work did not include an analysis of long non-coding RNAs (lncRNAs). Here, we applied a pipeline to identify and annotate lncRNAs in 66S. japonicum RNA-Seq publicly available libraries, from different life-cycle stages. We also performed co-expression analyses to find stage-specific lncRNAs possibly related to sexual maturation. We identified 12,291 S. japonicum expressed lnc RNAs. Sequence similarity search and synteny conservation indicated that some 14% of S. japonicum intergenic lncRNAs have synteny conservation withS. mansoni intergenic lncRNAs. Co-expression analyses showed that lncRNAs and protein-coding genes in S. japonicummales and females have a dynamic co-expression throughout sexual maturation, showing differential expression between the sexes; the protein-coding genes were related to the nervous system development, lipid and drug metabolism, and overall parasite survival. Co-expression pattern suggests that lncRNAs possibly regulate these processes or are regulated by the same activation program as that of protein-coding genes. (AU)

• Processo FAPESP: 14/03620-2 - Caracterização dos mecanismos de ação de RNAs longos não-codificadores envolvidos nos programas de ativação gênica em células humanas
• Beneficiário: Sergio Verjovski Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/19591-2 - Anotação de RNAs longos não-codificantes e identificação de potenciais alvos terapêuticos em Schistosoma mansoni
• Beneficiário: Lucas Ferreira Maciel
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/23693-5 - Mecanismos de ação de RNAs longos não-codificadores envolvidos nos programas de ativação gênica em eucariotos
• Beneficiário: Sergio Verjovski Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Temático