Functional and Mass Spectrometric Evaluation of an Anti-Tick Antigen Based on the P0 Peptide Conjugated to Bm86 Protein

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Rodriguez Mallon, Alina ^[1] ; Javier Gonzalez, Luis ^{[2, 3]} ; Encinosa Guzman, Pedro Enrique ^[1] ; Henrique Bechara, Gervasio ^{[4, 5]} ; Seron Sanches, Gustavo ^{[4, 5]} ; Pousa, Satomy ^{[2, 3]} ; Cabrera, Gleysin ^{[2, 3]} ; Cabrales, Ania ^[6] ; Garay, Hilda ^[6] ; Mejias, Raul ^[7] ; Lopez Alvarez, Jose Raul ^[7] ; Bello Soto, Yamil ^[1] ; Almeida, Fabiola ^{[2, 3]} ; Guirola, Osmany ^[8] ; Rodriguez Fernandez, Rafmary ^[9] ; Fuentes Castillo, Alier ^[9] ; Mendez, Luis ^[9] ; Jimenez, Samanta ^[10] ; Licea-Navarro, Alexei ^[10] ; Portela, Madelon ^{[11, 12]} ; Duran, Rosario ^{[11, 12]} ; Pablo Estrada, Mario ^[1]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Ctr Genet Engn & Biotechnol CIGB, Anim Biotechnol Dept, Havana 10600 - Cuba

^[2] CIGB, Mass Spectrometry Lab, Dept Prote, Havana 10600 - Cuba

^[3] CIGB, GlycoLab, Dept Prote, Havana 10600 - Cuba

^[4] Pontificia Univ Catolica Parana PUCPR, Programa Posgrad Ciencia Anim, BR-80215901 Curitiba, Parana - Brazil

^[5] Univ Estadual Paulista FCAV UNESP, Fac Ciencias Agr & Vet, Dept Patol Vet, BR-14884900 Sao Paulo - Brazil

^[6] CIGB, Synthet Peptides Grp, Havana 10600 - Cuba

^[7] Inst Ciencia Anim ICA, San Jose De Las Lajas 32700 - Cuba

^[8] CIGB, Bioinformat Dept, Havana 10600 - Cuba

^[9] Natl Lab Parasitol, San Antonio De Los Banos 32500 - Cuba

^[10] CICESE, Dept Innovac Biomed, Ensenada 22860, Baja California - Mexico

^[11] Inst Pasteur Montevideo, Unidad Bioquim & Prote Analit, Montevideo 11400 - Uruguay

^[12] Inst Invest Biol Clemente Estable IIBCE, Montevideo 11600 - Uruguay

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS; v. 9, n. 6 JUN 2020.
• Citações Web of Science: 1

Resumo

A synthetic 20 amino acid peptide of the ribosomal protein P0 from ticks, when conjugated to keyhole limpet hemocyanin fromMegathura crenulataand used as an immunogen againstRhipicephalus microplusandRhipicephalus sanguineuss.l. species, has shown efficacies of around 90%. There is also experimental evidence of a high efficacy of this conjugate againstAmblyomma mixtumandIxodes ricinusspecies, which suggest that this antigen could be a good broad-spectrum anti-tick vaccine candidate. In this study, the P0 peptide (pP0) was chemically conjugated to Bm86 as a carrier protein. SDS-PAGE analysis of this conjugate demonstrated that it is highly heterogeneous in size, carrying from 1 to 18 molecules of pP0 per molecule of Bm86. Forty-nine out of the 54 lysine residues and the N-terminal end of Bm86 were found partially linked to pP0 by using LC-MS/MS analysis and the combination of four different softwares. Several post-translational modifications of Bm86 protein were also identified by mass spectrometry. High immunogenicity and efficacy were achieved when dogs and cattle were vaccinated with the pP0-Bm86 conjugate and challenged withR. sanguineuss.l. andR. microplus, respectively. These results encourage the development of this antigen with promising possibilities as an anti-tick vaccine. (AU)

• Processo FAPESP: 13/10394-6 - Potencial Imunogênico da Proteína Ribossomal P0 Contra o Carrapato Rhipicephalus sanguineus (Acari:Ixodidae)em Cães
• Beneficiário: Gustavo Seron Sanches
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado