Serum and Muscle(1)H NMR-Based Metabolomics Profiles Reveal Metabolic Changes Influenced by a Maternal Leucine-Rich Diet in Tumor-Bearing Adult Offspring Rats

Autor(es)

da Silva Miyaguti, Natalia Angelo ^[1] ; Stanisic, Danijela ^[2] ; Pereira de Oliveira, Sarah Christine ^[1] ; dos Santos, Gabriela Sales ^[1] ; Manhe, Beatriz Schincariol ^[1] ; Tasic, Ljubica ^[2] ; Cintra Gomes-Marcondes, Maria Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Biol Inst, Dept Struct & Funct Biol, Lab Nutr & Canc, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Chem, Organ Chem Dept, Chem Biol Lab, Rua Josue Castro S-N, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRIENTS; v. 12, n. 7 JUL 2020.
• Citações Web of Science: 0

Resumo

A maternal leucine-rich diet showed a positive effect on the gastrocnemius muscle of adult tumor-bearing offspring. To improve the understanding of the metabolic alterations of cancer cachexia and correlate this to preventive treatment, we evaluated the(1)H NMR metabolic profiles from serum and gastrocnemius muscle samples of adult Wistar rats. These profiles were initially analyzed, and chemometrics tools were applied to investigate the following groups: C, control group; W, tumor-bearing group; L, the group without tumors and with a maternal leucine-rich diet; WL, the tumor-bearing group with a maternal leucine-rich diet. Tumor growth that led to a high protein breakdown in the W group was correlated to serum metabolites such as tyrosine, phenylalanine, histidine, glutamine, and tryptophan amino acids and uracil. Also, decreased muscle lactate, inversely to serum content, was found in the W group. Conversely, in the WL group, increased lactate in muscle and serum profiles was found, which could be correlated to the maternal diet effect. The muscle lipidomics and NAD(+), NADP(+), lysine, 4-aminohippurate, and glutamine metabolites pointed to modified energy metabolism and lower muscle mass loss in the WL group. In conclusion, this exploratory metabolomics analyses provided novel insights related to the Walker-256 tumor-bearing offspring metabolism modified by a maternal leucine-rich diet and the next steps in its investigation. (AU)

• Processo FAPESP: 19/13937-7 - Nutrição e câncer. estudo dos aspectos moleculares, proteômicos e metabolômicos em modelo experimental de caquexia
• Beneficiário: Natália Miyaguti Angelo da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/10809-2 - Avaliação do perfil de Metilação do DNA nos tecidos muscular e tumoral em ratos adultos, portadores do carcinossarcoma de Walker 256, provenientes da prole de ratas submetidas à dieta com óleo de fígado de bacalhau
• Beneficiário: Sarah Christine Pereira de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/02739-4 - Nutrição e câncer: estudo dos aspectos moleculares, proteômicos e metabolômicos em modelo experimental de caquexia
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/13334-7 - Nutrição e câncer: avaliação do perfil metabolômico, proteômico e das vias de sinalização celular muscular, placentária e fetal e do tecido tumoral sob influência dos hormônios da gravidez em ratas
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Regular