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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Serum and Muscle(1)H NMR-Based Metabolomics Profiles Reveal Metabolic Changes Influenced by a Maternal Leucine-Rich Diet in Tumor-Bearing Adult Offspring Rats

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da Silva Miyaguti, Natalia Angelo [1] ; Stanisic, Danijela [2] ; Pereira de Oliveira, Sarah Christine [1] ; dos Santos, Gabriela Sales [1] ; Manhe, Beatriz Schincariol [1] ; Tasic, Ljubica [2] ; Cintra Gomes-Marcondes, Maria Cristina [1]
Total Authors: 7
[1] Univ Campinas UNICAMP, Biol Inst, Dept Struct & Funct Biol, Lab Nutr & Canc, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Chem, Organ Chem Dept, Chem Biol Lab, Rua Josue Castro S-N, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: NUTRIENTS; v. 12, n. 7 JUL 2020.
Web of Science Citations: 0

A maternal leucine-rich diet showed a positive effect on the gastrocnemius muscle of adult tumor-bearing offspring. To improve the understanding of the metabolic alterations of cancer cachexia and correlate this to preventive treatment, we evaluated the(1)H NMR metabolic profiles from serum and gastrocnemius muscle samples of adult Wistar rats. These profiles were initially analyzed, and chemometrics tools were applied to investigate the following groups: C, control group; W, tumor-bearing group; L, the group without tumors and with a maternal leucine-rich diet; WL, the tumor-bearing group with a maternal leucine-rich diet. Tumor growth that led to a high protein breakdown in the W group was correlated to serum metabolites such as tyrosine, phenylalanine, histidine, glutamine, and tryptophan amino acids and uracil. Also, decreased muscle lactate, inversely to serum content, was found in the W group. Conversely, in the WL group, increased lactate in muscle and serum profiles was found, which could be correlated to the maternal diet effect. The muscle lipidomics and NAD(+), NADP(+), lysine, 4-aminohippurate, and glutamine metabolites pointed to modified energy metabolism and lower muscle mass loss in the WL group. In conclusion, this exploratory metabolomics analyses provided novel insights related to the Walker-256 tumor-bearing offspring metabolism modified by a maternal leucine-rich diet and the next steps in its investigation. (AU)

FAPESP's process: 19/13937-7 - Nutrition and Câncer. Molecular, Proteomic and Metabolomic aspects in experimental model of cachexia.
Grantee:Natália Miyaguti Angelo da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/10809-2 - Evaluation of DNA methylation profile in muscle and tumour tissue in adult rats, walker 256 tumour bearing, from offspring of rats submitted to COD liver oil diet
Grantee:Sarah Christine Pereira de Oliveira
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/02739-4 - Nutrition and cancer: study of molecular, proteomic and metabolomic aspects of experimental model of cachexia
Grantee:Maria Cristina Cintra Gomes Marcondes
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/13334-7 - Nutrition and cancer: metabolomic, and proteomic profile of muscle, placental, fetal, and tumor cell signaling pathways in Walker-256 tumour-bearing pregnant rats submitted to leucine nutritional supplementation
Grantee:Maria Cristina Cintra Gomes Marcondes
Support type: Regular Research Grants