Evaluation of DNA methylation profile in muscle and tumour tissue in adult rats, walker 256 tumour bearing, from offspring of rats submitted to COD liver oil diet

• Grant number: 17/10809-2
• Support type: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2018
• Effective date (End): August 31, 2019
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Cristina Cintra Gomes Marcondes
• Grantee: Sarah Christine Pereira de Oliveira
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 18/11932-5 - Molecular mechanisms of ovarian cancer prevention: investigating the epigenetic consequences of oral contraceptive use, BE.EP.MS

Abstract

The cancer pathology is especially aggravated when associated with cachexia. This condition frequently lead the patient to a high muscle spoliation (induced by the ubiquitin-proteasome proteolytic pathway and reduced protein synthesis), with or without loss of adipose tissue (resulted by higher lipolysis and total lipid oxidation ratio combined to lower lipogenesis). To prevent or modulate cachexia, nutritional supplementation is widely studied as an alternative, in which the polyunsaturated fatty acid omega-3 is highlighted, by its anti-inflammatory role, protein catabolism attenuation and genomic DNA methylation, reducing the appearance of different types of cancer. The cancer incidence is related to environment and lifestyle, then, the maintenance of a balanced diet throughout life and, especially, during the gestational and weaning period are effective strategies for cancer prevention or modulation of its harmful effects. Thus, nutriepigenetics could lead to minimise the global hypomethylation and the hypermethylation of specific genes promoters,possibly associated with protein synthesis and degradation, processes commonly deregulated in tumour development. So,our hypothesis is that nutritional supplementation with cod liver oil may promote conservation of body mass by regulation of protein synthesis and degradation in skeletal muscle, modulating the cachectic state led by cancer. Associating the omega-3 to pregnancy, this project aims to analyse the protein synthesis and degradation pathways in muscle of adulthood animals submitted to Walker 256 tumour development, whose mothers will receive this nutritional supplementation in pregnancy and weaning periods, and also the methylation of genes promoters involved in this pathways, as well as the global methylation in tumour. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA MIYAGUTI, NATALIA ANGELO; STANISIC, DANIJELA; PEREIRA DE OLIVEIRA, SARAH CHRISTINE; DOS SANTOS, GABRIELA SALES; MANHE, BEATRIZ SCHINCARIOL; TASIC, LJUBICA; CINTRA GOMES-MARCONDES, MARIA CRISTINA. Serum and Muscle(1)H NMR-Based Metabolomics Profiles Reveal Metabolic Changes Influenced by a Maternal Leucine-Rich Diet in Tumor-Bearing Adult Offspring Rats. NUTRIENTS, v. 12, n. 7 JUL 2020. Web of Science Citations: 0.

DA SILVA MIYAGUTI, NATALIA ANGELO; PEREIRA DE OLIVEIRA, SARAH CHRISTINE; CINTRA GOMES-MARCONDES, MARIA CRISTINA. Maternal Leucine-Rich Diet Minimises Muscle Mass Loss in Tumour-bearing Adult Rat Offspring by Improving the Balance of Muscle Protein Synthesis and Degradation. BIOMOLECULES, v. 9, n. 6 JUN 2019. Web of Science Citations: 0.