Biochemical changes in Leishmania braziliensis after photodynamic therapy with methylene blue assessed by the Fourier transform infrared spectroscopy

Autor(es)

Sakane, Kumiko Koibuchi ^[1] ; Bhattacharjee, Tanmoy ^[2] ; Fagundes, Jaciara ^[3] ; Marcolino, Luciana Maria Cortez ^[3] ; Ferreira, Isabelle ^{[3, 4]} ; Pinto, Juliana Guerra ^[3] ; Ferreira-Strixino, Juliana ^[3]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba Univap, Res & Dev Inst R&DI, Infrared Spect Lab, Shishima Hifumi Ave 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] Sir John Walsh Res Inst, 310 Great King St, Dunedin 9016 - New Zealand

^[3] Univ Vale Paraiba, Univap, Photobiol Appl Hlth Lab, Res & Dev Inst PI&D, Shishima Hifumi Ave 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[4] Univ Paulista, Inst Ciencias Saude ICS, Rod Presidente Dutra, Km 157, 5 Rio Comprido, BR-12240420 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 36, n. 4 AUG 2020.
• Citações Web of Science: 0

Resumo

Photodynamic therapy (PDT) with photosensitizer methylene blue was applied toLeishmania braziliensis, and Fourier transform infrared (FTIR) spectroscopy was used to study biochemical changes in the parasite after PDT in comparison to untreated (C), only irradiation (I), and only photosensitizer (PS). Spectral analysis suggests increase in lipids, proteins, and protein secondary structures in PDT compared with C and decrease in nucleic acids and carbohydrates. Interestingly, these trends are different from PDT ofLeishmania majorspecies, wherein lipids decrease; there are minimal changes in secondary structures and increase in nucleic acids and carbohydrates. The study thus suggests possibility of different biomolecular players/pathways in PDT-induced death ofL. braziliensisandL. major. (AU)

• Processo FAPESP: 16/12211-4 - Estudo do efeito genotóxico, do potencial de membrana e das vias de morte celular da curcumina na terapia fotodinâmica em promastigota e amastigota de L. major e L. braziliensis: estudo in vitro
• Beneficiário: Juliana Ferreira Strixino
• Modalidade de apoio: Auxílio à Pesquisa - Regular