Shedding the Light onLitopenaeus vannameiDifferential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

Autor(es)

Santos, Camilla A. ^{[1, 2]} ; Andrade, Sonia C. S. ^[1] ; Fernandes, Jorge M. O. ^[3] ; Freitas, Patricia D. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-676 Sao Carlos - Brazil

^[3] Nord Univ, Fac Biosci & Aquaculture, N-8026 Bodo - Norway

• Tipo de documento: Artigo Científico
• Fonte: GENES; v. 11, n. 7 JUL 2020.
• Citações Web of Science: 1

Resumo

White Spot Syndrome Virus (WSSV) is one of the main threats to farmingLitopenaeus vannamei, the most important crustacean commercialized in aquaculture worldwide. Here, we performed RNA-seq analyses in hepatopancreas and muscle from WSSV-negative (healthy) and WSSV-positive (unhealthy)L. vannamei,previously exposed to the virus, to obtain new insights about the molecular basis of resistance to WSSV. We detected 71% of our reads mapped against the recently describedL. vannameigenome. This is the first report mapping RNA-seq transcripts from shrimps exposed to WSSV against the species reference genome. Differentially expressed gene (DEG) analyses were performed for four independent comparisons, and 13,338 DEGs were identified. When the redundancies and isoforms were disregarded, we observed 8351 and 6514 DEGs, respectively. Interestingly, after crossing the data, we detected a common set of DEGs for hepatopancreas and healthy shrimps, as well as another one for muscle and unhealthy shrimps. Our findings indicate that genes related to apoptosis, melanization, and the Imd pathway are likely to be involved in response to WSSV, offering knowledge about WSSV defense in shrimps exposed to the virus but not infected. These data present potential to be applied in further genetic studies in penaeids and other farmed shrimp species. (AU)

• Processo FAPESP: 12/17322-8 - Anotação genômica e validação populacional de SSR-EST e SNP-EST para identificação de genes candidatos no camarão marinho Litopenaeus vannamei (Penaeidae, Decapoda)
• Beneficiário: Patrícia Domingues de Freitas
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/13069-6 - Anotação genômica e validação populacional de SSR-EST e SNP-EST para identificação de genes candidatos no camarão marinho Litopenaeus vannamei (Penaeidae, Decapoda)
• Beneficiário: Camilla Alves Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado