Derivatization-free determination of aminoglycosides by CZE-UV in pharmaceutical formulations

Autor(es)

Donegatti, Tiago Augusto ^[1] ; Lobato, Alnilan ^[2] ; Duek, Eliana A. R. ^[3] ; Goncalves, Luis Moreira ^[2] ; Pereira, Elisabete Alves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos UFSCar, Dept Fis Quim & Matemat, Rodovia Joao Leme dos Santos, Km 110, SP 264, BR-18052780 Sorocaba, SP - Brazil

^[2] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Sao Paulo, SP - Brazil

^[3] Pontifical Univ Catholic Sao Paulo PUC SP, Med & Hlth Sci Fac, Biomat Lab, Sorocaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ELECTROPHORESIS; v. 41, n. 18-19 AUG 2020.
• Citações Web of Science: 1

Resumo

Aminoglycosides are a relevant class of antibiotics widely used by medics and veterinaries. There are a variety of reasons that make their determination relevant, such as quality control, environment and food contamination assessment, drug-release studies, among others. The lack of a chromophore makes aminoglycoside spectrophotometric detection particularly challenging, often requiring derivatization. In this work, an indirect detection method, making use of imidazole as a probe, applying CZE was successfully tested. It did not require derivatization, which simplified the sample preparation. Suitable figures of merit were obtained; recoveries between 95 and 105%, adequate repeatability and precision, correlation coefficients (r) above 0.998, and limits of detection (LODs) of 3.2 and 11 mg/L for gentamicin and paromomycin, respectively. As a proof-of-concept, it was also applied in a simple controlled release experiment that was well fitted using the Hill equation. (AU)

• Processo FAPESP: 18/14425-7 - Um novo rumo sinérgico para a microextração por difusão gasosa (GDME) fazendo uso de polímeros com impressão molecular (MIPs)
• Beneficiário: Luís Francisco Moreira Gonçalves
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/03582-7 - Eletroforese capilar com técnica analítica para a determinação de aminas biogênicas em matrizes de alimentos
• Beneficiário: Elisabete Alves Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/11214-8 - Recurso à eletroforese capilar após extração por GDME-MIPs para a determinação de aldeídos
• Beneficiário: Alnilan Cristina Barros Lobato
• Modalidade de apoio: Bolsas no Brasil - Mestrado