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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Derivatization-free determination of aminoglycosides by CZE-UV in pharmaceutical formulations

Full text
Author(s):
Donegatti, Tiago Augusto [1] ; Lobato, Alnilan [2] ; Duek, Eliana A. R. [3] ; Goncalves, Luis Moreira [2] ; Pereira, Elisabete Alves [1]
Total Authors: 5
Affiliation:
[1] Univ Fed Sao Carlos UFSCar, Dept Fis Quim & Matemat, Rodovia Joao Leme dos Santos, Km 110, SP 264, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Sao Paulo, SP - Brazil
[3] Pontifical Univ Catholic Sao Paulo PUC SP, Med & Hlth Sci Fac, Biomat Lab, Sorocaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ELECTROPHORESIS; v. 41, n. 18-19 AUG 2020.
Web of Science Citations: 1
Abstract

Aminoglycosides are a relevant class of antibiotics widely used by medics and veterinaries. There are a variety of reasons that make their determination relevant, such as quality control, environment and food contamination assessment, drug-release studies, among others. The lack of a chromophore makes aminoglycoside spectrophotometric detection particularly challenging, often requiring derivatization. In this work, an indirect detection method, making use of imidazole as a probe, applying CZE was successfully tested. It did not require derivatization, which simplified the sample preparation. Suitable figures of merit were obtained; recoveries between 95 and 105%, adequate repeatability and precision, correlation coefficients (r) above 0.998, and limits of detection (LODs) of 3.2 and 11 mg/L for gentamicin and paromomycin, respectively. As a proof-of-concept, it was also applied in a simple controlled release experiment that was well fitted using the Hill equation. (AU)

FAPESP's process: 18/14425-7 - A new synergetic path for gas-diffusion microextraction (GDME) making use of molecular imprinted polymers (MIPs)
Grantee:Luís Francisco Moreira Gonçalves
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 19/03582-7 - Capillary electrophoresis as analytical technique for the determination of biogenic amines in food matrices
Grantee:Elisabete Alves Pereira
Support Opportunities: Regular Research Grants
FAPESP's process: 19/11214-8 - Application of capillary electrophoresis for the determination of aldehydes after extraction by GDME-MIPs
Grantee:Alnilan Cristina Barros Lobato
Support Opportunities: Scholarships in Brazil - Master