Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis

Autor(es)

Francisconi, Renata Serignoli ^[1] ; Maquera-Huacho, Patricia Milagros ^[1] ; Tonon, Caroline Coradi ^[1] ; Fioramonti Calixto, Giovana Maria ^[2] ; Orlandi Sardi, Janaina de Cassia ^[3] ; Chorilli, Marlus ^[2] ; Palomari Spolidorio, Denise Madalena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Sch Dent Araraquara, Dept Physiol & Pathol, UNESP, Araraquara, SP - Brazil

^[2] UNESP, Sch Pharmaceut Araraquara, Dept Drugs & Med, Araraquara, SP - Brazil

^[3] Univ Estadual Campinas, Sch Dent Piracicaba, Dept Physiol Sci, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 JUL 31 2020.
• Citações Web of Science: 0

Resumo

This study was performed to develop a liquid crystalline system (LCS) incorporated with terpinen-4-ol and nystatin to evaluate its antifungal, antibiofilm, and synergistic/modulatory activity against Candida albicans. The LCS was composed of a dispersion containing 40% propoxylated and ethoxylated cetyl alcohol, 40% oleic acid, and 0.5% chitosan dispersion. According to analysis by polarized light microscopy, rheology, and mucoadhesion studies, the incorporation of 100% artificial saliva increased the pseudoplasticity, consistency index, viscosity, and mucoadhesion of the formulation. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity; the LCS containing terpinen-4-ol and nystatin effectively inhibited C. albicans growth at a lower concentration, displaying a synergistic action. Therefore, LCS incorporated with terpinen-4-ol and nystatin is a promising alternative for preventing and treating infections and shows potential for the development of therapeutic strategies against candidiasis. (AU)

• Processo FAPESP: 15/08742-1 - Caracterização do terpinen-4-ol sobre fungos e bactérias: avaliação do efeito sinérgico/aditivo, citotoxicidade, adesão em co-cultura e alteração dos fatores de virulência
• Beneficiário: Denise Madalena Palomari Spolidorio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/23241-6 - Avaliação dos fatores de virulência e viabilidade celular de bactérias cariogênicas na presença do Terpinen-4-ol: estudo in vitro
• Beneficiário: Ester Alves Ferreira Bordini Galvani
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários