Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis

Author(s)

Francisconi, Renata Serignoli ^[1] ; Maquera-Huacho, Patricia Milagros ^[1] ; Tonon, Caroline Coradi ^[1] ; Fioramonti Calixto, Giovana Maria ^[2] ; Orlandi Sardi, Janaina de Cassia ^[3] ; Chorilli, Marlus ^[2] ; Palomari Spolidorio, Denise Madalena ^[1]

Affiliation

^[1] Univ Estadual Paulista, Sch Dent Araraquara, Dept Physiol & Pathol, UNESP, Araraquara, SP - Brazil

^[2] UNESP, Sch Pharmaceut Araraquara, Dept Drugs & Med, Araraquara, SP - Brazil

^[3] Univ Estadual Campinas, Sch Dent Piracicaba, Dept Physiol Sci, Piracicaba, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 10, n. 1 JUL 31 2020.
• Web of Science Citations: 0

Abstract

This study was performed to develop a liquid crystalline system (LCS) incorporated with terpinen-4-ol and nystatin to evaluate its antifungal, antibiofilm, and synergistic/modulatory activity against Candida albicans. The LCS was composed of a dispersion containing 40% propoxylated and ethoxylated cetyl alcohol, 40% oleic acid, and 0.5% chitosan dispersion. According to analysis by polarized light microscopy, rheology, and mucoadhesion studies, the incorporation of 100% artificial saliva increased the pseudoplasticity, consistency index, viscosity, and mucoadhesion of the formulation. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity; the LCS containing terpinen-4-ol and nystatin effectively inhibited C. albicans growth at a lower concentration, displaying a synergistic action. Therefore, LCS incorporated with terpinen-4-ol and nystatin is a promising alternative for preventing and treating infections and shows potential for the development of therapeutic strategies against candidiasis. (AU)

• FAPESP's process: 15/08742-1 - Characterization of Terpinen-4-ol on fungi and bacteria: evaluation of synergic effect/additive, cytotoxicity, adhesion in co-culture and change of virulence factors
• Grantee: Denise Madalena Palomari Spolidorio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/23241-6 - Evaluation of virulence factors and cell viability of cariogenic bacteria in the presence of Terpinen-4-ol: in vitro study
• Grantee: Ester Alves Ferreira Bordini Galvani
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
• Grantee: Igor Polikarpov
• Support Opportunities: Multi-user Equipment Program