Uncovering Biological Application of Brazilian Green Propolis: A Phenotypic Screening againstSchistosoma mansoni

Autor(es)

A. de L. Paula, Lucas ^[1] ; Santos, Mario F. C. ^[1] ; Pagotti, Mariana C. ^[1] ; Faleiros, Renata ^[1] ; Ramos, Henrique P. ^[1] ; Veneziani, Rodrigo C. S. ^[1] ; Bastos, Jairo K. ^[2] ; Caffrey, Conor R. ^[3] ; Ambrosio, Sergio R. ^[1] ; Magalhaes, Lizandra G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Ctr Res Sci & Technol, Res Grp Nat Prod, Ave Dr Armando Salles Oliveira, BR-14404600 Franca, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, Ctr Discovery & Innovat Parasit Dis, 9500 Gilman Dr, La Jolla, CA 92093 - USA

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRY & BIODIVERSITY; v. 17, n. 9 AUG 2020.
• Citações Web of Science: 2

Resumo

The chemotherapy of schistosomiasis remains centered in the use of praziquantel, however, there has been growing resistant parasites to this drug. Thus, the aim of this work was to evaluatein vitroschistosomicidal activity of the hexanes/dichloromethane 1 : 1 extract of Brazilian green propolis (Pex), as well as its major isolated compounds artepillin C, caffeic acid, coumaric acid and drupanin againstSchistosoma mansoni. The Pex was active by displaying an IC(50)value of 36.60 (26.26-51.13) mu g mL(-1)at 72 h against adult worms ofS. mansoni. The major isolated compounds were inactive with IC(50)values >100 mu M, however, the combination of the isolated compounds (CM) in the same range found in the extract was active with an IC(50)value of 41.17 (39.89-42.46) mu g mL(-1)at 72 h. Pex and CM induced alteration in the tegument ofS. mansoni, and caffeic acid caused alteration in egg's maturation. Pex displayedin vitroactivity against adult worms' and eggs' viability ofS. mansoni, which opens new perspectives to better understand the synergistic and/or additive effects promoted by both Pex extract and CM against schistosomiasis features. (AU)

• Processo FAPESP: 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/50011-2 - Exploring turmeric curcuminoids to treat schistosomiasis: an evaluation of their pre-clinical potential
• Beneficiário: Lizandra Guidi Magalhães
• Modalidade de apoio: Auxílio à Pesquisa - Regular