Solid-liquid extraction of cellulases from fungal solid-state cultivation in a packed bed bioreactor

Autor(es)

de Oliveira, Samuel Pratavieira ^[1] ; Rodrigues, Natalia Alvarez ^[1] ; Casciatori-Frassatto, Priscila Aparecida ^[2] ; Casciatori, Fernanda Perpetua ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Dept Chem Engn, Rodovia Washington Luiz Km 235 SP 310, BR-13565905 Sao Carlos, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Chem & Environm Sci, Inst Biosci Letters & Exact Sci, Cristovao Colombo 2265, 15054-000 15054000, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: KOREAN JOURNAL OF CHEMICAL ENGINEERING; v. 37, n. 9 AUG 2020.
• Citações Web of Science: 0

Resumo

Cellulases are enzymes required for the production of second-generation ethanol (E2G) via biochemical route. The current paper reports the development of an apparatus for solid-liquid extraction of cellulases from solid-state fermentation (SSF) carried out in a packed bed bioreactor (PBB), operated as batch and as semicontinuous. The case study was the cultivation ofMyceliophthora thermophilaI-1D3b on sugarcane bagasse (SCB) and wheat bran (WB) (7: 3 w/w). The current work integrates the PBB to the first downstream step for recovering the enzymes produced by SSF. The substrate was inoculated and packed into the modules that composed the PBB. The fermentation occurred at 45 degrees C and air was supplied with flow rate of 350 L/h. At the end of the cultivations, each module was placed in an extraction column, a dynamic closed system in which distilled water was circulated and made to percolate the cultivated material. Variables tested were volume of water per mass of substrate, water flow rate and time of percolation. Higher contact time (120 min) and higher flow rate (2.4m(3)/h) allowed us to recover up to 85% of total enzyme activity by percolation. Lower volume (20mL/g) provides higher titer extract. The apparatus showed to be promising for SSF downstream, mainly for semicontinuous operation of PPBs. (AU)

• Processo FAPESP: 18/26097-4 - Desenvolvimento de sistema de extração de celulases produzidas por fermentação em estado sólido em biorreator de leito empacotado
• Beneficiário: Samuel Pratavieira de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/16689-1 - Produção de celulases em biorrefinarias por fermentação em estado sólido em biorreatores de leito empacotado
• Beneficiário: Natalia Alvarez Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Mestrado