Solid-liquid extraction of cellulases from fungal solid-state cultivation in a packed bed bioreactor

Author(s)

de Oliveira, Samuel Pratavieira ^[1] ; Rodrigues, Natalia Alvarez ^[1] ; Casciatori-Frassatto, Priscila Aparecida ^[2] ; Casciatori, Fernanda Perpetua ^[1]

Affiliation

^[1] Fed Univ Sao Carlos UFSCar, Dept Chem Engn, Rodovia Washington Luiz Km 235 SP 310, BR-13565905 Sao Carlos, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Chem & Environm Sci, Inst Biosci Letters & Exact Sci, Cristovao Colombo 2265, 15054-000 15054000, SP - Brazil

• Document type: Journal article
• Source: KOREAN JOURNAL OF CHEMICAL ENGINEERING; v. 37, n. 9 AUG 2020.
• Web of Science Citations: 0

Abstract

Cellulases are enzymes required for the production of second-generation ethanol (E2G) via biochemical route. The current paper reports the development of an apparatus for solid-liquid extraction of cellulases from solid-state fermentation (SSF) carried out in a packed bed bioreactor (PBB), operated as batch and as semicontinuous. The case study was the cultivation ofMyceliophthora thermophilaI-1D3b on sugarcane bagasse (SCB) and wheat bran (WB) (7: 3 w/w). The current work integrates the PBB to the first downstream step for recovering the enzymes produced by SSF. The substrate was inoculated and packed into the modules that composed the PBB. The fermentation occurred at 45 degrees C and air was supplied with flow rate of 350 L/h. At the end of the cultivations, each module was placed in an extraction column, a dynamic closed system in which distilled water was circulated and made to percolate the cultivated material. Variables tested were volume of water per mass of substrate, water flow rate and time of percolation. Higher contact time (120 min) and higher flow rate (2.4m(3)/h) allowed us to recover up to 85% of total enzyme activity by percolation. Lower volume (20mL/g) provides higher titer extract. The apparatus showed to be promising for SSF downstream, mainly for semicontinuous operation of PPBs. (AU)

• FAPESP's process: 18/26097-4 - Development of a system for extraction of cellulases produced by solid-state fermentation in packed-bed bioreactor
• Grantee: Samuel Pratavieira de Oliveira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/16689-1 - Cellulases production in biorefineries by solid-state fermentation in packed-bed bioreactors
• Grantee: Natalia Alvarez Rodrigues
• Support Opportunities: Scholarships in Brazil - Master