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Influence of nutrient solution on microbial production of citric acid from sugarcane bagasse

Grant number: 17/24460-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2018
Effective date (End): February 28, 2019
Field of knowledge:Biological Sciences - Biochemistry - Biochemistry of Microorganisms
Principal Investigator:Reinaldo Gaspar Bastos
Grantee:Laura Macedo Rocha
Home Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil

Abstract

Solid-state cultivation (SSC) is characterized by growth in solid supports, agroindustrial by-products for example, under limited water conditions. Due to these characteristics, filamentous fungi are the most used microorganisms to obtain the various metabolites potentiated in this type of culture. Regarding the inoculum, the use of fungal consortia has been studied with the main advantage being the complementation in terms of microbial hydrolases, which leads to a better utilization of the substrates contained in the solid supports. Citric acid is a bioproduct with several industrial applications, and its conventional production occurs from aerobic submerged cultures in saccharose-medium. However, its production from solid supports, such as sugarcane bagasse, has been the objective of several studies, and the accumulation of citric acid can be metabolically induced by non-limited oxygen conditions and by the presence of lower alcohols, mainly methanol and ethanol. In this context, the present research intends to evaluate the production of citric acid by CES from the consortium of Trichoderma reesei and Aspergillus niger from sugarcane bagasse impregnated with vinasse and ethanol in packed-bed bioreactors at two different scales. Considering that previous studies by our research group at the Center of Agricultural Sciences (CCA) of UFSCar indicate feasibility since type of SSC, this proposal expands the finding for the use of microbial consortia and study of a prescreening in column bioreactors, with aspects of consumption and oxygen transfer not addressed in these systems. The academic study returns to the evaluation of an unconventional SSC from mixed cultures and adding two by-products generated in the same industrial platform, meeting the demands of the sugarcane processing industry, important in the region of Araras where the CCA/UFSCar is located. (AU)