| Texto completo | |
| Autor(es): Mostrar menos - |
Britto-Junior, Jose
[1]
;
Pinheiro, David H. A.
[1]
;
Justo, Alberto F. O.
[1]
;
Figueiredo Murari, Guilherme M.
[1]
;
Campos, Rafael
[2]
;
Mariano, V, Fernanda
;
de Souza, Valeria B.
[1]
;
Schenka, Andre A.
[1]
;
Monica, Fabiola Z.
[1]
;
Antunes, Edson
[1]
;
De Nucci, Gilberto
[3, 1, 4]
Número total de Autores: 11
|
| Afiliação do(s) autor(es): | [1] State Univ Campinas Unicamp, Fac Med Sci, Dept Pharmacol, Campinas - Brazil
[2] Ceara State Univ UECE, Super Inst Biomed Sci, Fortaleza, Ceara - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
[4] Brazil Univ, Fernadopolis - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PHARMACOLOGY RESEARCH & PERSPECTIVES; v. 8, n. 4 AUG 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Electrical field stimulation (EFS) induces contractions of both snake aorta and human umbilical cord vessels (HUCV) which were dependent on the presence of the endothelium. This study aimed to establish the nature of the mediator(s) responsible for EFS-induced contractions in HUCV. Rings with or without endothelium from human umbilical artery (HUA) or vein (HUV) were mounted in organ bath chambers containing oxygenated, heated Krebs-Henseleit's solution. Basal release of dopamine (DA), noradrenaline, and adrenaline was measured by LC-MS-MS. Cumulative concentration-response curves were performed with dopamine in the absence and in the presence of L-NAME or of dopamine antagonists. EFS studies were performed in the presence and absence of L-NAME, the alpha-adrenergic blockers prazosin and idazoxan, and the dopamine antagonists SCH-23390 and haloperidol. Tyrosine hydroxylase (TH) and dopa-decarboxylase (DDC) were studied by immunohistochemistry and fluorescence in situ hybridizations. Basal release of dopamine requires an intact endothelium in both HUA and HUV. TH and DDC are present only in the endothelium of both HUA and HUV as determined by immunohistochemistry. Dopamine induced contractions in HUA only in the presence of L-NAME. Dopamine-induced contractions in HUV were strongly potentiated by L-NAME. The EFS-induced contractions in both HUA and HUV were potentiated by L-NAME and inhibited by the D2-like receptor antagonist haloperidol. The alpha-adrenergic antagonists prazosin and idazoxan and the D1-like receptor antagonist SCH-23390 had no effect on the EFS-induced contractions of HUA and HUV. Endothelium-derived dopamine is a major modulator of HUCV reactivityin vitro. (AU) | |
| Processo FAPESP: | 17/15175-1 - Modulação da guanilato ciclase solúvel e dos níveis intracelulares de nucleotídeos cíclicos em órgãos do trato urinário inferior e próstata |
| Beneficiário: | Edson Antunes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |