| Texto completo | |
| Autor(es): |
Regiart, Matias
[1]
;
Gimenez, Alba Marina
[2]
;
Lopes, Alexandre T.
[3]
;
Carreno, Marcelo N. P.
[3]
;
Bertotti, Mauro
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Polytech Sch, Dept Elect Syst Engn, BR-05508010 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Analytica Chimica Acta; v. 1127, p. 122-130, AUG 29 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
An ultrasensitive and portable microfluidic electrochemical immunosensor for SOX-2 cancer biomarker determination was developed. The selectivity and sensitivity of the sensor were improved by modifying the microfluidic channel. This was accomplished through a physical-chemical treatment to produce a hydrophilic surface, with an increased surface to volume/ratio, where the anti-SOX-2 antibodies can be covalently immobilized. A sputtered gold electrode was used as detector and its surface was activated by using a dynamic hydrogen bubble template method. As a result, a gold nanoporous structure (NPAu) with outstanding properties, like high specific surface area, large pore volume, uniform nanostructure, good conductivity, and excellent electrochemical activity was obtained. SOX-2 present in the sample was bound to the anti-SOX-2 immobilized in the microfluidic channel, and then was labeled with a second antibody marked with horseradish peroxidase (HRP-anti-SOX-2) like a sandwich immunoassay. Finally, an H2O2 + catechol solution was added, and the enzymatic product (quinone) was reduced on the NPAu electrode at +0.1 V (vs. Ag). The current obtained was directly proportional to the SOX-2 concentration in the sample. The detection limit achieved was 30 pg mL(-1), and the coefficient of variation was less than 4.75%. Therefore, the microfluidic electrochemical immunosensor is a suitable clinical device for in situ SOX-2 determination in real samples. (C) 2020 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais |
| Beneficiário: | Mauro Bertotti |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/06293-6 - Investigações em sistemas biológicos empregando sensores eletroquímicos miniaturizados |
| Beneficiário: | Daniel Matias Gaston Regiart |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |