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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ultrasensitive microfluidic electrochemical immunosensor based on electrodeposited nanoporous gold for SOX-2 determination

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Author(s):
Regiart, Matias [1] ; Gimenez, Alba Marina [2] ; Lopes, Alexandre T. [3] ; Carreno, Marcelo N. P. [3] ; Bertotti, Mauro [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Polytech Sch, Dept Elect Syst Engn, BR-05508010 Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Analytica Chimica Acta; v. 1127, p. 122-130, AUG 29 2020.
Web of Science Citations: 0
Abstract

An ultrasensitive and portable microfluidic electrochemical immunosensor for SOX-2 cancer biomarker determination was developed. The selectivity and sensitivity of the sensor were improved by modifying the microfluidic channel. This was accomplished through a physical-chemical treatment to produce a hydrophilic surface, with an increased surface to volume/ratio, where the anti-SOX-2 antibodies can be covalently immobilized. A sputtered gold electrode was used as detector and its surface was activated by using a dynamic hydrogen bubble template method. As a result, a gold nanoporous structure (NPAu) with outstanding properties, like high specific surface area, large pore volume, uniform nanostructure, good conductivity, and excellent electrochemical activity was obtained. SOX-2 present in the sample was bound to the anti-SOX-2 immobilized in the microfluidic channel, and then was labeled with a second antibody marked with horseradish peroxidase (HRP-anti-SOX-2) like a sandwich immunoassay. Finally, an H2O2 + catechol solution was added, and the enzymatic product (quinone) was reduced on the NPAu electrode at +0.1 V (vs. Ag). The current obtained was directly proportional to the SOX-2 concentration in the sample. The detection limit achieved was 30 pg mL(-1), and the coefficient of variation was less than 4.75%. Therefore, the microfluidic electrochemical immunosensor is a suitable clinical device for in situ SOX-2 determination in real samples. (C) 2020 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
Grantee:Mauro Bertotti
Support type: Research Projects - Thematic Grants
FAPESP's process: 19/06293-6 - Investigations in biological systems by using miniaturized electrochemical sensors
Grantee:Daniel Matias Gaston Regiart
Support type: Scholarships in Brazil - Post-Doctorate