The molecular insights into protein adsorption on hematite surface disclosed by in-situ ATR-FTIR/2D-COS study

Autor(es)

Barreto, Matheus Sampaio C. ^{[1, 2]} ; Elzinga, Evert J. ^[3] ; Alleoni, Luis Reynaldo F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Dept Soil Sci, Piracicaba, SP - Brazil

^[2] Mohammed VI Polytech Univ UM6P, AgroBiosci Div, Lot 660, Hay Moulay Rachid 43150, Benguerir - Morocco

^[3] Rutgers State Univ, Dept Earth & Environm Sci, Newark, NJ - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 AUG 10 2020.
• Citações Web of Science: 0

Resumo

Proteins are of ubiquitous interest in the Life Sciences but are of interest in the Geosciences as well because of the significant role these compounds play in the biogeochemical cycling of trace and nutrient elements. Structural changes resulting from the adsorption of proteins onto mineral surfaces may alter protein biological function and other environmental interactions. Iron oxides are major sinks of a range of environmental elements including organic compounds. In this study, the adsorption of the broadly studied model protein BSA onto the hematite mineral surface was characterized as a function of pH, ionic strength, and BSA concentration using in-situ Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy. BSA lost the alpha-helix and gain beta-sheets in the secondary structure during adsorption on hematite. BSA adsorption was maximum at pH 5, a value close to the BSA isoelectric point (similar to pH 5), and lower at pH 4 and pH 7. Increasing ionic strength decreased to total BSA adsorption. Two-dimensional correlation spectroscopy analysis of the ATR-FTIR spectra revealed that higher initial BSA concentration and the consequent higher BSA surface loading enhanced BSA adsorption by protein-protein interaction, which less ordered structures changes into more compact forms decrease, hence compacting the structural arrangement and could promoting multilayers/aggregation formation on the mineral surface. The activity of enzymes following adsorption on mineral surfaces requires further study. (AU)

• Processo FAPESP: 16/05870-1 - ESPECIAÇÃO E CINÉTICA DE ADSORÇÃO E DESSORÇÃO DE Cd E DE Zn EM SOLOS DE ÁREAS OLERÍCOLAS ALTAMENTE FERTILIZADAS
• Beneficiário: Matheus Sampaio Carneiro Barreto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/22058-9 - Adsorção e dessorção de Cd e Zn em solos tropicais altamente fertilizados
• Beneficiário: Matheus Sampaio Carneiro Barreto
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado