Rhythms of Transcription in Field-Grown Sugarcane Are Highly Organ Specific

Autor(es)

de Barros Dantas, Luiza Lane ^{[1, 2]} ; Almeida-Jesus, Felipe Marcelo ^[1] ; de Lima, Natalia Oliveira ^[1] ; Alves-Lima, Cicero ^[1] ; Nishiyama-, Jr., Milton Yutaka ^[3] ; Carneiro, Monalisa Sampaio ^[4] ; Souza, Glaucia Mendes ^[1] ; Hotta, Carlos Takeshi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil

^[2] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam - Germany

^[3] Inst Butantan, Lab Especial Toxicol Aplicada, BR-05503900 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Biotecnol & Prod Vegetal & Anim, Ctr Ciencias Agr, BR-13600970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 APR 16 2020.
• Citações Web of Science: 3

Resumo

Circadian clocks improve plant fitness in a rhythmic environment. As each cell has its own circadian clock, we hypothesized that sets of cells with different functions would have distinct rhythmic behaviour. To test this, we investigated whether different organs in field-grown sugarcane follow the same rhythms in transcription. We assayed the transcriptomes of three organs during a day: leaf, a source organ; internodes 1 and 2, sink organs focused on cell division and elongation; and internode 5, a sink organ focused on sucrose storage. The leaf had twice as many rhythmic transcripts (>68%) as internodes, and the rhythmic transcriptomes of the internodes were more like each other than to those of the leaves. Among the transcripts expressed in all organs, only 7.4% showed the same rhythmic pattern. Surprisingly, the central oscillators of these organs - the networks that generate circadian rhythms - had similar dynamics, albeit with different amplitudes. The differences in rhythmic transcriptomes probably arise from amplitude differences in tissue-specific circadian clocks and different sensitivities to environmental cues, highlighted by the sampling under field conditions. The vast differences suggest that we must study tissue-specific circadian clocks in order to understand how the circadian clock increases the fitness of the whole plant. (AU)

• Processo FAPESP: 11/00818-8 - Desenvolvimento de modelos biológicos alternativos para o estudo de redes de regulação em cana-de-açúcar
• Beneficiário: Carlos Takeshi Hotta
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores
• Processo FAPESP: 16/06740-4 - Caracterização do relógio biológico em tecidos fotossintéticos de plantas C4
• Beneficiário: Natalia Oliveira de Lima
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/08897-4 - Caracterização do relógio biológico e seu impacto no metabolismo da cana-de-açúcar
• Beneficiário: Luíza Lane de Barros Dantas
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/05301-9 - Estudo sobre as interações entre o relógio biológico e a dessecação em eucariotos fotossintetizantes.
• Beneficiário: Cícero Alves Lima Júnior
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/06260-0 - Relógios biológicos específicos em órgãos e tecidos de gramíneas C4
• Beneficiário: Carlos Takeshi Hotta
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular