Comparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms

Autor(es)

de Campos, Bruna K. ^{[1, 2]} ; Galazzi, Rodrigo M. ^{[1, 2]} ; dos Santos, Bruna M. ^[3] ; Balbuena, Tiago S. ^[3] ; dos Santos, Fabio N. ^[4] ; Mokochinski, Joao B. ^[5] ; Eberlin, Marcos N. ^[4] ; Arruda, Marco A. Z. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Spectrometry Sample Preparat & Mechanizat Grp GEP, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Natl Inst Sci & Technol Bioanalyt, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

^[3] State Univ Julio de Mesquita Filho UNESP, Sch Agr & Vet Studies, Dept Technol, BR-14884900 Jaboticabal, SP - Brazil

^[4] Univ Campinas UNICAMP, Inst Chem, Dept Organ Chem, POB 6154, BR-13083970 Campinas, SP - Brazil

^[5] Imperial Coll London, London Inst Med Sci, Fac Med, UK Res & Innovat, London W12 0NN - England

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 202, OCT 1 2020.
• Citações Web of Science: 0

Resumo

This work evaluates different generations of transgenic (cp4-EPSPS gene) and non-transgenic soybean plants through proteomics and metabolomics. For proteomics purpose, 24 differentially abundant protein spots were found through 2-D DIGE, being 4 belonging to transgenic plants. From this total, 19 were successfully identified, storage proteins as predominant class. Some identified proteins are involved in growing and cell division, and stress response, such as LEA and dehydrin. For metabolomics, 17 compounds were putatively annotated, mainly belonging to the secondary metabolism, such as flavonoids. From these analyzes, all generations and varieties of the soybean are prone to be differentiate by PLS-DA. According to our results, transgenic plants appear to be more stable than non-transgenic ones. In addition, the omics-based approaches allowed access some relations between those differential spot proteins and metabolites, mainly those storage proteins and flavonoid. (AU)

• Processo FAPESP: 13/17514-7 - Comparação de Possíveis Alterações Protéicas em Sementes e Plantas de Soja Transgênica e Não-Transgênica Provenientes de Distintas Gerações
• Beneficiário: Bruna Kauely de Campos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/25207-0 - Nanometalômica aplicada ao cultivo da soja para estudar os efeitos das nanopartículas relacionadas a metal
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/07384-7 - Bioimageamento elementar por meio da ablação a laser e espectrometria de massas com plasma indutivamente acoplado
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/15646-3 - Avaliação de plantas de soja transgênica e não-transgênica cultivadas em solo tratado com nanopartículas de prata.
• Beneficiário: Rodrigo Moretto Galazzi
• Modalidade de apoio: Bolsas no Brasil - Doutorado