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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms

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de Campos, Bruna K. [1, 2] ; Galazzi, Rodrigo M. [1, 2] ; dos Santos, Bruna M. [3] ; Balbuena, Tiago S. [3] ; dos Santos, Fabio N. [4] ; Mokochinski, Joao B. [5] ; Eberlin, Marcos N. [4] ; Arruda, Marco A. Z. [1, 2]
Total Authors: 8
[1] Univ Campinas UNICAMP, Spectrometry Sample Preparat & Mechanizat Grp GEP, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Natl Inst Sci & Technol Bioanalyt, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil
[3] State Univ Julio de Mesquita Filho UNESP, Sch Agr & Vet Studies, Dept Technol, BR-14884900 Jaboticabal, SP - Brazil
[4] Univ Campinas UNICAMP, Inst Chem, Dept Organ Chem, POB 6154, BR-13083970 Campinas, SP - Brazil
[5] Imperial Coll London, London Inst Med Sci, Fac Med, UK Res & Innovat, London W12 0NN - England
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 0

This work evaluates different generations of transgenic (cp4-EPSPS gene) and non-transgenic soybean plants through proteomics and metabolomics. For proteomics purpose, 24 differentially abundant protein spots were found through 2-D DIGE, being 4 belonging to transgenic plants. From this total, 19 were successfully identified, storage proteins as predominant class. Some identified proteins are involved in growing and cell division, and stress response, such as LEA and dehydrin. For metabolomics, 17 compounds were putatively annotated, mainly belonging to the secondary metabolism, such as flavonoids. From these analyzes, all generations and varieties of the soybean are prone to be differentiate by PLS-DA. According to our results, transgenic plants appear to be more stable than non-transgenic ones. In addition, the omics-based approaches allowed access some relations between those differential spot proteins and metabolites, mainly those storage proteins and flavonoid. (AU)

FAPESP's process: 13/17514-7 - Comparison of Possible Alterations in protein Soybean Seeds and Plants Transgenic and Non-Transgenic Coming from Distinct Generations
Grantee:Bruna Kauely de Campos
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/25207-0 - Nanometallomics applied to soybean cultivation for studying the effects of metal-related nanoparticles
Grantee:Marco Aurelio Zezzi Arruda
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/07384-7 - Elementary bioimaging through laser ablation and inductively coupled plasma mass spectrometry
Grantee:Marco Aurelio Zezzi Arruda
Support type: Regular Research Grants
FAPESP's process: 13/15646-3 - Evaluation of transgenic and non-transgenic soybean plants cultivated in soil treated with silver nanoparticles.
Grantee:Rodrigo Moretto Galazzi
Support type: Scholarships in Brazil - Doctorate