Quantification of differential proteins between transgenic and non-transgenic soybean seeds using tandem mass spectrometry (label free) approach

• Grant number: 15/24668-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): April 01, 2016
• Effective date (End): July 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Marco Aurelio Zezzi Arruda
• Grantee: Bruna Kauely de Campos
• Supervisor: Jay J. Thelen
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Research place: University of Missouri, Columbia (UM), United States
• Associated to the scholarship: 13/17514-7 - Comparison of Possible Alterations in protein Soybean Seeds and Plants Transgenic and Non-Transgenic Coming from Distinct Generations, BP.DR

Abstract

The following proposal for the Research Internship Abroad Fellowship - BEPE is within the context of my PhD project supervised by FAPESP. Here we are proposing the proteomic quantification approach using high resolution mass spectrometry specifically Orbitrap technology. This technology is considered worldwide as the best one for protein identification. In Brazil there are no group with the enough expertise for developing our proposal as Thelen's group has, including quantification with spectra counting only and using high resolution mass spectrometry. Additionally, this group has a long experience with biochemistry of oil seeds and plants as well as in the development of data management strategies focusing on proteomics datasets. Furthermore, they offer a combination of multiple analytical platforms fully supported by biochemical, bioinformatics and biostatistics expertise, which is not found in Brazilian research centers concerning plant proteomics. Thus, it is easy to rationalize that such proposal complements the PhD research project developed by our research group in Brazil, covering a complete study of the soybean seed proteomic as well as comparing the technology used for both quantification and identification of proteins. Additionally, a diversity of analytical chemistry concepts can also be applied for improving proteins identification and quantification, and due to our expertise will be certainly focused/apprimorated. (AU)