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In-vitro bioaccessibility study of some important biological metals in transgenic and non-transgenic soybean

Grant number: 13/02809-1
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2013
Effective date (End): July 31, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Marco Aurelio Zezzi Arruda
Grantee:Monica Alejandra Herrera Agudelo
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):14/19369-7 - In vitro bioaccessibility of transgenic and non-transgenic soybeans using automated microdialysis-based system and filtration, BE.EP.MS

Abstract

This project consists in the study of bioaccessibility of metals in transgenic and non-transgenic soybean (Glycinemax (L). Merril) intending establishes possible differences caused by genetic modification. The bioaccessibility is evaluated using in-vitro gastrointestinal digestion methods that can be static or dynamic, which simulate the human-digestion process phases. The in-vitro static models perform a sequential exposure to mimic digestion in different areas of the human digestive system (mouth, stomach, intestine and combinations of them). Thus, the aim of this work is to determine the bioacessible fractions in the transgenic and non-transgenic soybean obtained after the application of an in-vitro static gastrointestinal digestion method and to verify the influence of the genetic modification in the bioaccessibility of Cu, Fe, Mn and Zn. Also, human physiological conditions will be evaluated as pH, gastric and intestinal enzymes, temperature and residence time. Initially, it will be performed the total quantification of Cu, Fe, Mn and Zn in transgenic and non-transgenic soybean seeds by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Thereafter, the behavior of these elements after extraction will be evaluated using sequential fractionation in order to determine the distribution of these elements in transgenic and non-transgenic soybeans, if in hydrophobic or hydrophilic media. The second stage of the experimental work will comprise the bioaccessibility study of the elements after a simulated grastrointestinal digestion procedure (in-vitro) in order to establish the amount of minerals that have been released from the matrix seeds. (AU)