Full text
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| Author(s): |
Mónica Alejandra Herrera Agudelo
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Química |
| Defense date: | 2015-07-30 |
| Examining board members: |
Marco Aurelio Zezzi Arruda;
Amauri Antônio Menegario;
José Alberto Fracassi da Silva
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| Advisor: | Marco Aurelio Zezzi Arruda |
| Abstract | |
The soy (Glicinemax(L). Merril) is one of the most important crops and with highest economic worth in the world, among other characteristics, it has excellent nutritional qualities, is widespread throughout the world and is part of the alimentary habits of humans and animals. At present, the most of its production uses transgenic soybeans. However, there is a lot to know about the nutritional differences between the transgenic variety and not transgenic. That is why this work consist in the study of the differentiation, in terms of bioaccesibility, of the metals between transgenic and non transgenic soybean, through a comparative nutritional study. Soybeans seeds of the varieties MSOY8200 (NT, non transgenic) and M7211RR (T, transgenic) are cultivated in different seasons (precursors soybean seeds, winter and summer), and compared through the total content of Cu, Fe, Mn and Zn. In the correlation between the crops of the same season, some significant statistical differences in the concentration of the metals are found between the T and NT soybeans. For the evaluation of the bioaccessibility, a unified method of gastrointestinal simulation, in vitro UBM, was used in order to obtain the gastric and gastrointestinal bioaccessibility fraction. In the gastric fraction the highest percentage of bioaccessible fraction of the analytes is obtained, from 106% (Mn), 38% (Fe) in NT soybeans seeds and 107% (Mn), 26% (Fe) for T soybean seeds. However, in the gastrointestinal conditions a decrease on the percentage of the soluble fraction of Fe (9-14%), Mn (27-41%) and Zn (7-38%) is observed, while for Cu the highest percentages of bioaccessibility (73-98%) is found. Finally, a study of the bioaccessibility in T and NT soybeans seeds, is implemented using a sequential injection analysis system based on microfiltration for on-line detection by ICP OES, which is proposed by the first time. In this system the kinetic profiles of the gastrointestinal extractions of the metals studied for soybean seeds cultivated in summer are obtined. The recoveries for Cu, Fe and Mn have variations from 87% to 118% in NT soybean seeds and from 95% to 100% for T soybean seeds. This procedure is prone in evaluating the gastrointestinal bioaccessibility of the analytes in the T and NT soybean seeds (AU) | |
| FAPESP's process: | 13/02809-1 - In-vitro bioaccessibility study of some important biological metals in transgenic and non-transgenic soybean |
| Grantee: | Monica Alejandra Herrera Agudelo |
| Support Opportunities: | Scholarships in Brazil - Master |