Comparative omics through hyphenated techniques focusing on metal complexes in transgenic and non-transgenic soybean after cultivation with silver nanoparticles.

• Grant number: 16/18373-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): November 01, 2016
• Effective date (End): April 30, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Marco Aurelio Zezzi Arruda
• Grantee: Rodrigo Moretto Galazzi
• Supervisor: Joanna Szpunar
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Research place: Université de Pau et des Pays de l'Adour, France
• Associated to the scholarship: 13/15646-3 - Evaluation of transgenic and non-transgenic soybean plants cultivated in soil treated with silver nanoparticles., BP.DR

Abstract

The following proposal for the Research Internship Abroad Fellowship - BEPE is within the context of my PhD project sponsored by FAPESP. As proposal, will be employed hyphenated techniques, such as liquid chromatography coupled to ICP-MS, and high-resolution MS, as Orbitrap technology, for evaluating some important metal complexes (metallobiomolecules), to which their abundances or concentrations will be differentially found, comparing transgenic (T) and non-transgenic (NT) soybean leaves cultivated under the presence of AgNP or AgNO3. Moreover, this bioinorganic speciation can also bring additional information concern the biochemical pathways involved in the Ag detoxification, once were recently observed evidences about Ag absorption by soybean plants in our study. In addition, the group of Prof. Szpunar has a vast experience with metallomic approaches regarding bioinorganic speciation, employing a variety of strategies focused on hyphenated platforms, especially multidimensional chromatography coupled to ICP-MS, and high-resolution MS to identify such metallobiomolecules. This expertise, combined with excellent facilities, is not found in Brazilian research centers concerning plant metallomics.Therefore, this proposal will complement my PhD research project developed in the Prof. Arruda research group in Brazil, covering an additional and complementary information about T and NT soybean plants cultivated under the presence of AgNP or AgNO3. Additionally, a diversity of analytical chemistry concepts combined with our expertise, especially concerning the sample preparation for biomolecules, can also be applied for improving the separation, quantification and identification of the metal complexes.