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Evaluation of isotopic distribution of 57Fe in different parts of transgenic and non-transgenic soybean plants (Glicine max L. Merril) by ICP-MS and mapping by means of images using LA-ICP-MS

Grant number: 11/12054-2
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): October 01, 2011
Effective date (End): September 30, 2013
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Marco Aurelio Zezzi Arruda
Grantee:Silvana Ruella de Oliveira
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In the last years, the cultivation and utilization of transgenic soybean have been greatly increasing. However, there is still little information about the effects that the genetic modification may bring to nutritional quality of the soybean. Aiming evaluate if the Fe distribution in the transgenic soybean may suffer some kind of change, it is intended to cultivate transgenic and non-transgenic soybeans under the same conditions and enrich them with the isotope 57Fe. Thus, after the harvest and separation of transgenic and non-transgenic soybean plants parts (seed, stem, root and leaves), it is intended to quantify the isotope 57Fe in that parts by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). By means of bi-dimensional images of this isotope employing LA-ICP-MS, it will be possible diagnose the probable changes in its distribution in the different compartments of the plant. The investigation of the most abundant isotope 56Fe in the transgenic and non-transgenic soybean plants will be studied as well. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA, SILVANA R.; ARRUDA, MARCO A. Z. Application of laser ablation (imaging) inductively coupled plasma mass spectrometry for mapping and quantifying Fe in transgenic and non-transgenic soybean leaves. Journal of Analytical Atomic Spectrometry, v. 30, n. 2, p. 389-395, 2015. Web of Science Citations: 15.
OLIVEIRA, SILVANA R.; MENEGARIO, AMAURI A.; ARRUDA, MARCO A. Z. Evaluation of Fe uptake and translocation in transgenic and non-transgenic soybean plants using enriched stable Fe-57 as a tracer. METALLOMICS, v. 6, n. 10, p. 1832-1840, 2014. Web of Science Citations: 6.

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