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Comparative Metallomics of transgenic and non-transgenic Arabidopsis thaliana´s leaves using 2D-HPLC-ICP-MS and ESI-MS/MS

Grant number: 13/02810-0
Support type:Scholarships in Brazil - Master
Effective date (Start): June 01, 2013
Effective date (End): May 31, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Marco Aurelio Zezzi Arruda
Grantee:Katherine Chacón Madrid
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


The objective of this research is to differentiate transgenic and non-transgenic Arabidopsis thaliana´s leaves in terms of seleno-proteomic. This work will use 2D-HPLC-ICP-MS/MS for quantification and identification of selenium in protein´s leaves. Initially, proteins of Arabidopsis thaliana´s leaves will be extracted by using phenol extraction method, thereafter, the protein mass will be determined from Bradford´s method. Chromatographic separations of protein will be developed using a size exclusion chromatography (SEC) as first dimension, and then a second dimension will be included by using anion exchange chromatography (AIX). The identification of seleno-proteins previously separated will be made by UPLC-ESI-MS/MS.

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