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| Author(s): |
Katherine Chacón Madrid
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Química |
| Defense date: | 2015-07-29 |
| Examining board members: |
Marco Aurelio Zezzi Arruda;
Ricardo Antunes Azevedo;
Alessandra Sussulini
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| Advisor: | Marco Aurelio Zezzi Arruda |
| Abstract | |
In order to evaluate the effects of selenium (Se) and/or the genetic modification in plants metabolism, oxidative stress and resistance of Se accumulation studies and also metalomics analysis are performed using, Arabidopsis thaliana leaves, genetically modified (MG) and unmodified (N-MG), in a greenhouse and irrigated with sodium selenite. MG and N-MG plants show some differences due to the presence of selenite. The N-MG plants when irrigated with the highest dose of Se (308 µg), accumulate 17% less Se and had 5% more reduction in their growth (reflected in low amount of fresh organic mass of leaves), comparing with MG plants. In addition, when N-MG control plants are compared with those treated with Se, a reduction of up to 10% in the SOD activity and an increase of 162% in MDA concentration are observed. Moreover, when the highest dose of Se is used, MG leaves show a significant increase (48 %) in SOD activity and production of H2O2, and a decrease of 11 % on the MDA concentration. However, the concentration of soluble protein in the extract of N-MG leaves is more than 33% higher compared with MG leaves, and no difference in the presence of selenite, while the MG leaves show a decrease of 32% in the highest concentration of Se. The SEC-DAD-ICP-MS system, allows the monitoring of the increase in the chromatographic peaks of selenium species soluble in water in N-MG A. thaliana leaves, irrigated with sodium selenite solution when it is compared with MG leaves data. Results of studies of MG and N-MG A. thaliana leaves, under different treatment conditions, allow to evaluate not only the genetic modification, but also the presence of selenium, which both effects play an important role in plant metabolism, such as a higher production of reactive oxygen species. The genetic modification also confers an increased on the plants resistance against the oxidative stress produced by the presence of selenite. Moreover, variations in the group of hydrophilic compounds, for instance, proteins, as possible identify between the A. thaliana MG and N-MG leaves control and irrigated with a solution of sodium selenite (AU) | |
| FAPESP's process: | 13/02810-0 - Comparative Metallomics of transgenic and non-transgenic Arabidopsis thaliana´s leaves using 2D-HPLC-ICP-MS and ESI-MS/MS |
| Grantee: | Katherine Chacón Madrid |
| Support Opportunities: | Scholarships in Brazil - Master |